摘要
为推动海洋生物质资源可持续开发,并探索陆生胶原蛋白的可替代来源,以野生真海鞘为原料,采用酸酶结合法提取胶原蛋白,并与以牛跟腱为原料提取的胶原进行对比,分析探讨海洋生物源胶原的应用潜力。通过凝胶电泳、紫外吸收光谱、氨基酸组成分析、Zeta电位测试、差示扫描量热分析、红外吸收光谱和扫描电镜观察等手段对其结构进行系统研究。结果表明,所提取的真海鞘胶原在120 ku左右有2条α链(α1和α2),在200 ku左右有一条β链,紫外吸收峰在230 nm处,符合Ⅰ型胶原特征且胶原三螺旋结构保留完整。真海鞘胶原与牛跟腱胶原相比含有更多的谷氨酸等呈味氨基酸,约占氨基酸总量的43.07%。真海鞘胶原等电点约为5.9,热变性温度为28.08℃。扫描电镜结果显示真海鞘胶原海绵内部呈多层聚集、疏松多孔、表面光滑的片状交织结构,与牛跟腱胶原类似。真海鞘胶原有望成为陆生胶原的特色替代来源,在食品、医药及化妆品领域有良好的应用潜力。
In order to promote the sustainable development of marine biomass resources and explore alternative sources of terrestrial collagen,collagen from wild Halocynthia roretzi(HR)was extracted by an acid-enzyme combined method,and compared with collagen extracted from bovine achilles tendon(BAT),to analyze and explore the application potential of marine biological source collagen.Various analytical techniques were employed for systematic structural studies,including sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)absorption spectroscopy,amino acid composition analysis,isoelectric point(PI)test,differential scanning calorimetry(DSC),Fourier transform infrared spectroscopy(FT-IR)and scanning electron microscopy(SEM).The results showed that the collagen from HR exhibited twoα-chains(α1 andα2)around 120 ku and oneβ-chain around 200 ku.The ultraviolet absorption peak was at 230 nm,which was consistent with the characteristics of type I collagen,and the triple helical structure of collagen was well preserved.Compared to BAT collagen,HR collagen contained more flavored amino acids such as glutamic acid,accounting for 43.07%of the total amino acids.The PI of HR collagen was about 5.9,and the denaturation temperature was 28.08℃.SEM results revealed that the internal structure of HR collagen sponges consists of multilayered aggregation,loose porosity,smooth surface flake interweaving structure,similar to BAT collagen.These findings suggest that HR collagen has the potential to serve as a special alternative source to terrestrial collagen and demonstrates promising application in the food,pharmaceuticals and cosmetics industries.
作者
王越
程宽
易一帆
汪燕
张敏
刘学平
丁翠翠
WANG Yue;CHENG Kuan;YI Yifan;WANG Yan;ZHANG Min;LIU Xueping;DING Cuicui(College of Ecological Environment and Urban Construction,Fujian University of Technology,Fuzhou 350118,China;College of Materials Engineering,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《食品科技》
CAS
北大核心
2023年第9期210-216,共7页
Food Science and Technology
基金
国家自然科学基金面上项目(22078060)
福建工程学院科研启动基金项目(GY-Z160002)。
关键词
真海鞘
胶原蛋白
海洋生物质
酸酶法
理化性质
Halocynthia roretzi
collagen
marine biomass
acid enzyme method
physicochemical properties
