摘要
目的:探索新疆石河子市汉维两族有龋和无龋学龄前儿童口腔菌群差异。方法:纳入符合标准的54名3~5岁儿童并分为4组:汉族无龋组(15例)和患龋组(14例),维族无龋组(13例)和患龋组(12例)。收集其非刺激性的唾液,通过16S rDNA测序分析其口腔菌群差异。结果:汉维两族有龋组和无龋组相比:门水平上,拟杆菌门物种相对丰度均较高。属水平上,普氏菌属相对丰度均较高。维吾尔族无龋组和汉族无龋组相比:门水平上,拟杆菌门物种丰度较高而硬壁菌门物种丰度较低;属水平上,普氏菌属丰度较低而韦荣球菌属丰度较高。α多样性分析:维吾尔族有龋组Chao1指数和PD whole tree指数均低于无龋组(P<0.05)。β多样性分析:不同分组间存在组内部分聚集部分离散,组间部分聚集及不同位置分布。结论:汉维两族学龄前儿童,同一民族内在无龋和有龋状态下口腔细菌物种丰度存在差异。对于维吾尔族学龄前儿童而言,患龋儿童的口腔细菌多样性较少。
Objective:To explore the difference of oral flora between Han and Uygur preschool children with and without caries in Shihezi,Xinjiang.Methods:54 children aged 3-5 years who met the criteria were recruited and divided into 4 groups:caries free group(n=15)and caries-affected group(n=14)of Han children,caries free group(n=13)and caries affected group(n=12)of Uygur children.Unstimulated saliva was collected and the oral microflora was analyzed by 16S rDNA sequencing.Results:In the same nationality,when compared with caries-free groups,the relative abundance in Prevotella and Bacteroidota in caries-affected groups were higher.Compared with the caries-free group of the Han nationality,the relative abundance of Bacteroidota and Veillonella in the caries-free group of Uygur nationality were higher,while Firmicutes and Prevotella were lower.Alpha diversity analysis showed that the Chao1 index and PD whole tree index of the Uygur group with caries were lower than those of the caries free group(P<0.05).Beta diversity analysis showed that there were partial aggregation within groups and partial dispersion between groups,with distribution and different locations between groups.Conclusion:There is a difference in the abundance of oral bacteria species of preschool children between 2 ethnic groups.For Uyghur preschool children,the diversity of oral bacteria in children with caries is less.
作者
余重庆
沈玉凤
郑程东
陈铎
李东慧
钱艺
张若珍
马学花
周政
YU Chongqing;SHEN Yufeng;ZHENG Chengdong;CHEN Duo;LI Donghui;QIAN Yi;ZHANG Ruozhen;MA Xuehua;ZHOU Zheng(Department of Stomatology,The First Affiliated Hospital of Shihezi University Medical College,832000,China;Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases,College of Stomatology,Xi'an Jiaotong University)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2023年第6期756-761,共6页
Journal of Practical Stomatology
基金
“西安交通大学口腔医院陕西省牙颌疾病临床医学研究中心”开放课题(编号:2020YHJB02)
石河子大学青年创新人才计划项目(编号:CXPY202103)。
