摘要
比较了PF-EMF暴露下W1118野生型和Rye突变型果蝇发育、睡眠和运动节律行为的差异.连续暴露处理3d后去除亲本果蝇,记录子代发育过程并监测运动和睡眠节律行为.结果表明,PF-EMF暴露后W1118型成蛹量、成蝇量分别显著提高56.2%和57.5%,Rye型果蝇成蛹量无显著差异,成蝇量显著增加85.2%,子代雌雄比例未显著变化.从行为数据看,两株系果蝇暴露后运动和睡眠节律相位未见显著差异,W1118型表现为24h平均运动次数增加37.3%,24h睡眠片段次数减少10.6%,Rye型24h平均运动次数显著增加50.0%,24h睡眠片段次数减少51.5%,总睡眠时长显著降低45.7%.和W1118型相比,Rye型果蝇发育、运动、睡眠情况改变更显著,提示Rye型果蝇电磁暴露更为敏感.总之PF-EMF环境对昆虫发育和节律行为均可带来影响,造成后代数量上升、显著活跃果蝇运动行为并引起睡眠障碍.本研究为进一步了解电磁暴露对昆虫类生物的影响,合理评估PF-EMF的生态效应提供依据.
Differences in development,sleep and movement rhythmic behaviors between W1118 wild-type flies and Rye mutants were investigated under PF-EMF exposure.After continuous exposure for 3 days,the parental flies were removed.Developmental process,movement and sleep rhythmic behaviors of offspring were monitored.The results showed that,the pupal and fly population of W1118 significantly increased respectively by 56.2%and 57.5%under PF-EMF exposure.No significant changes were observed in pupal numbers of Rye type flies,but the fly population significantly increased by 85.2%.No significant change of sex ratio was found under PF-EMF exposure.For W1118 flies,the average number of 24-hour movements was increased by 37.3%after PF-EMF exposure,and 10.6%decrease in the number of 24-hour sleep segments was calculated.For Rye type flies,the average counts of 24-hour movement significantly increased by 50.0%after exposure,and 51.5%decrease of 24-hour sleep segment counts was investigated.In the meanwhile,45.7%decrease of the total sleep duration were also found.Compared with the W1118 type,the Rye mutants showed more significant changes,which suggested that Rye mutants were more sensitive to PF-EMF exposure.In brief,the PF-EMF environment can impact development and rhythmic behaviors of insects.Increased fly offspring numbers movement counts,and disturbed sleep were found after PF-EMF exposure.Further understandings on the entomological effects of PF-EMF exposure,and reasonable evaluations on the ecological effects of PF-EMF can be provided in this study.
作者
邹鑫平
王晓丽
端正花
张紫燕
孙永彦
ZOU Xin-ping;WANG Xiao-li;DUAN Zheng-hua;ZHANG Zi-yan;SUN Yong-yan(School of Environmental Science and Safety Engineering,Tianjin University of Technology,Tianjin 300384,China;Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China;Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,Shanghai 200031,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2023年第11期6149-6155,共7页
China Environmental Science
基金
自然资源部海洋生物遗传资源重点实验室开放课题项目(HY202106)
国家自然科学基金资助项目(32201017)。
关键词
工频电磁场
黑腹果蝇
节律行为
发育
PF-EMF
Drosophila melanogaster
behavioral rhythms
development
