摘要
以野生型黑腹果蝇(Drosophilamelanogaster)为基本实验材料,统计分析了非致死的冷、热预刺激对果蝇耐极限温度能力的影响,并用SDSPAGE电泳研究了冷、热刺激后热休克蛋白的表达情况.实验发现,冷、热预刺激能提高果蝇在同类温度下的耐受水平.同时,高、低温具有交互作用,即冷预刺激提高了果蝇在高温下的存活率.在0℃下,果蝇体内22、23、26、27、42、51、52及70kD的蛋白质出现增量表达;37℃下70、83kD的蛋白质表达量增多,推测上述增效作用可能是这些蛋白质引起的.
The effects of cold- and heat-hardening on resistance to both low and high temperature stress was examined in Drosophila melanogaster wild-type lines.The expression of heat shock proteins was detected using SDS-PAGE electrophoresis.The hardening effect was positive when the hardening was of the same type as the stress. A cross-protection effect was also present,which is increased heat resistance after cold hardening. When flies were exposed to 0℃, proteins of 22,23,26,27,42,51,52 and 70 kD increase expression,while heat shock at 37℃ induces the added expression of 70 kD and 83 kD proteins. The results suggest that protection-effect be at least partly due to the presence of above proteins.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第B06期186-190,共5页
Journal of Xiamen University：Natural Science
基金
教育部留学回国人员启动基金资助
