摘要
目的:采用非靶向代谢组学技术探究麸炒苍术多糖与麸炒苍术正丁醇部位对脾虚大鼠血浆代谢产物的影响,进而阐明二者健脾作用机制。方法:将40只雄性SD大鼠随机分为空白组、模型组、多糖组(0.075 6 g·mL^(-1))和正丁醇部位组(0.012 1 g·mL^(-1)),每组10只。除空白组外,其余3组采用过度劳倦、饮食失节加苦寒泻下的复合因素造模方法复制脾虚大鼠模型,造模结束后多糖组和正丁醇部位组分别给予相应药液灌胃治疗7 d,空白组和模型组给予等体积生理盐水。采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法(UHPLC-Q-Orbitrap-MS)分析空白组、模型组、多糖组与正丁醇部位组大鼠血浆样品,并采用多元统计分析筛选差异代谢产物;采用京都基因与基因组百科全书(KEGG)数据库结合MetaboAnalyst 5.0软件对筛选出的差异代谢物进行代谢通路分析。结果:多元统计分析结果显示,模型组与空白组、多糖组、正丁醇部位组间代谢产物均存在明显差异。空白组与模型组间共有380个差异代谢物,麸炒苍术多糖与麸炒苍术正丁醇部位分别回调了其中78个和57个差异代谢物。代谢通路富集结果显示,麸炒苍术正丁醇部位主要影响了甘氨酸、丝氨酸和苏氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢及D-精氨酸、D-鸟氨酸代谢;麸炒苍术多糖主要影响了甘氨酸、丝氨酸和苏氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,生物素代谢,硫胺素代谢及三羧酸循环。结论:麸炒苍术多糖与正丁醇部位均对脾虚大鼠的代谢异常有明显调节作用,其中正丁醇部位主要涉及氨基酸代谢,多糖除影响氨基酸代谢外,还参与调节能量代谢与辅因子和维生素代谢。
Objective:To explore the regulatory effect of polysaccharides and n-butanol fractions of Atractylodis Rhizoma stir-fried with bran on the plasma metabolites of spleen-deficient rats,and then to elucidate their mechanisms of spleen-enhancing effects.Method:Forty male SD rats were randomly divided into the blank group,model group,polysaccharide group(FD group,0.0756 g·mL^(-1)·d^(-1)),n-butanol fractions group(FZ group,0.0121 g·mL^(-1)·d^(-1)),with 10 rats in each group.Except the blank group,the other three groups used the compound factors of overwork,dietary disorders and intragastric administration of Sennae Folium decoction to replicate the rat model of spleen deficiency.After the end of modeling,the FD group and FZ group were given the corresponding medicinal solution by gavage for 7 d,meanwhile,the blank group and model group were given an equal volume of saline.The plasma samples from rats in the blank,model,FZ and FD groups were analyzed by ultra-high performance liquid chromatography-quadrupole/electrostatic fieldorbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS), multivariate statistical methods wereused to process the data and screen differential metabolites, and metabolic pathway enrichment analysis of thescreened differential metabolites was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG)database and Metabo Analyst 5.0. Result: The results of multivariate statistical analysis showed that there weresignificant differences in plasma metabolites between the model group and blank group, FZ group and modelgroup, FD group and model group. There were 380 differential metabolites between the blank group and themodel group, of which 78 and 57 were called back by polysaccharides and n-butanol fractions of AtractylodisRhizoma stir-fried with bran, respectively. Metabolic pathway enrichment results showed that the n-butanolfractions mainly affected glycine, serine and threonine metabolism, alanine, aspartate and glutamatemetabolism, D-arginine and D-ornithine metabolism, which w
作者
徐慧颖
丁奕辉
董昊
赵雪岑
柏阳
才谦
XU Huiying;DING Yihui;DONG Hao;ZHAO Xuecen;BAI Yang;CAI Qian(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2023年第22期112-120,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81973478)
兴辽英才计划项目(XLYC2002004)。
关键词
苍术
代谢组学
多糖
多元统计分析
生物标志物
氨基酸代谢
正丁醇部位
Atractylodis Rhizoma
metabonomics
polysaccharides
multivariate statistical analysis
biomarker
amino acid metabolism
n-butanol fractions
