摘要
为筛选珍珠龙胆石斑鱼有水保活过程中的生命质量特征指标,以石斑鱼有水保活过程中存活率、体质量损失率、肌肉的一般化学组成、质构特性、肌肉组织形态、血清生化指标、鱼鳃Na^(+)/K^(+)-ATP酶活性及其组织形态等为观测指标,探究不同有水保活时间(0、24、48、72、96、120 h)下珍珠龙胆石斑鱼肌肉品质及生理生化指标的变化。试验结果显示,随着保活时间的延长,珍珠龙胆石斑鱼的存活率降低、体质量损失率显著升高(P<0.05),肌肉中粗脂肪、粗蛋白含量显著降低(P<0.05),肌肉硬度也逐渐降低;肌肉组织形态发生了明显变化,肌纤维逐渐收缩、空隙逐渐增大;血清中谷丙转氨酶和谷草转氨酶活性逐渐变强,皮质醇含量先降后增;鱼鳃中Na^(+)/K^(+)-ATP酶活性呈先降后升趋势,鳃小片形状变长变细,并且伴有水肿现象,同时鱼鳃白度显著增加(P<0.05)。血清指标和鱼鳃组织形态有望成为表征石斑鱼生命质量特征的有效指标,该结果可为石斑鱼保活流通过程中生命质量特征的动态监测提供参考。
The survival rate,body mass loss rate,general chemical composition and texture of muscle characteristics,muscle tissue morphology,serum biochemical indicators,fish gill Na^(+)/K^(+)-ATPase activity and tissue morphology were determined in pearl gentian grouper(Epinephelus fuscoguttatus♂×E.lanceolatus♀)with a body mass of(700±50)g placed in a 90×45×30 cm tank with continuous oxygenation for 0,24,48,72,96 and 120 h at a density of 100 g/L at a salinity of 24-26 and water temperature of(25±2)℃to explore the changes in muscle quality and physiological and biochemical indicators at different water retention periods.The result showed that the survival rate of pearl gentian grouper was showed to be decreased,and body weight loss rate to be significantly increased(P<0.05),contents of crude fat and protein to be significantly decreased(P<0.05)with the time extended,while the hardness to be depressed gradually.With the extension of survival time,the morphology of muscles tissue was observed to be changed significantly,with increase in the gap between muscles fibers gradually,and the fractured structure of muscles tissue gradually.The activity of alanine aminotransferase and aspartate transaminase in fish blood was increased,while cortisol level went down firstly then up.There was decrease in Na^(+)/K^(+)-ATPase activity in gills and then increase.The lamellae became longer and thinner,accompanied by edema,and the whiteness of gills was increased significantly(P<0.05).The serum biochemical properties and the gills tissue morphology characteristics were expected to be potential indicators to characterize the of pearl gentian grouper during keeping\|alive,which provided reference for the dynamic monitoring of the quality of live grouper during the live with water.
作者
温敬敬
王蕾
王金厢
李学鹏
励建荣
丁玉庭
谢晶
王彦波
秦小明
WEN Jingjing;WANG Lei;WANG Jinxiang;LI Xuepeng;LI Jianrong;DING Yuting;XIE Jing;WANG Yanbo;QIN Xiaoming(National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,College of Food Science and Engineering,Bohai University,Jinzhou 121013,China;College of Food Science and Engineering,Zhejiang University of Technology,Hangzhou 310014,China;College of Food Science,Shanghai Ocean University,Shanghai 201306,China;School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China;College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China)
出处
《水产科学》
CAS
CSCD
北大核心
2023年第6期945-954,共10页
Fisheries Science
基金
国家重点研发计划项目(2019YFD0901605)。
关键词
珍珠龙胆石斑鱼
有水保活
肌肉品质
生理生化指标
生命质量特征
grouper hybrid Epinephelus fuscoguttatus[QX(Y12]♂[QX)]×E.lanceolatus♀
live with water
muscle quality
physiochemical property
life quality characteristic
