摘要
目的评估慢性高脂肪饮食(HFD)对缺血/再灌注(I/R)大鼠脑损伤影响及机制。方法60只雄性Wistar大鼠随机分为正常饮食(ND)组(n=30)和高脂肪饮食(HFD)组(n=30),每组再分别分为Sham组和I/R组两个亚组,每组均为15只大鼠。采用神经行为学法评估各组大鼠神经功能缺损;采用HE染色法检测各组大鼠脑组织病理学变化;采用尼氏染色法检测各组大鼠神经元存活率;采用Western blotting法检测各组大鼠脑组织细胞焦亡相关蛋白(NLRP3、Caspase-1、IL-1β)及HMGB1/TLR4/NF-κB信号通路蛋白表达。结果与ND组相比,HFD组大鼠体质量、内脏脂肪组织、甘油三酯、总胆固醇和葡萄糖水平均增加(P<0.05)。在I/R组中,与ND大鼠相比,HFD大鼠出现更严重神经功能缺损;HE染色显示HFD大鼠具有更多受损细胞;尼氏染色显示HFD大鼠存活神经元数量减少;HFD大鼠细胞焦亡相关蛋白(NLRP3、Caspase-1、IL-1β)表达较高;HFD大鼠HMGB1及其下游因子TLR4和NF-κB表达较高(P<0.05)。结论HFD摄入会加重大鼠脑I/R损伤后细胞焦亡,并增强HMGB1/TLR4/NF-κB信号通路表达,从而导致I/R后不良结局。
Objective To evaluate the effect of chronic high fat diet(HFD)on brain injury in ischemia/reperfusion(I/R)rats and its mechanisms.Methods Sixty male Wistar rats were randomly divided into normal diet(ND)group(n=30)and high fat diet(HFD)group(n=30).Each group was further divided into Sham group and I/R group,with 15 rats in each group.Neurobehavioral method was used to evaluate the neurological deficits of rats in each group;HE staining was used to detect the pathological changes of brain tissue of rats in each group;the survival rate of neurons in each group was measured by Nissl staining;western blotting method was used to detect the Pyroptosis-related proteins(NLRP3,Caspase-1,IL-1β),and HMGB1/TLR4/NF-κB signal pathway protein expression in brain tissue of rats in each group.Results Compared with the ND group,the body weight,visceral adipose tissue,triglyceride,total cholesterol and glucose water of rats in HFD group were increased(all P<0.05).In the I/R group,compared with the ND rats,HFD rats showed more severe neurological impairment;HE staining showed that HFD rats had more damaged cells;niss staining showed that the number of surviving neurons in HFD rats decreased;the expressions of pyroptosis related proteins(NLRP3,Caspase-1,IL-1β)were higher in HFD rats;high expression of HMGB1 and its downstream factors TLR4 and NF-κB in HFD rats(all P<0.05).Conclusion HFD intake could aggravate pyrodeath and enhance the expression of HMGB1/TLR4/NF-κB signaling pathway after brain I/R injury,leading to adverse outcomes after I/R.
作者
李加善
杨德兵
彭志锋
LI Jiashan;YANG Debin;PENG Zhifeng(Department of Anatomy,School of Medicine,Shanxi Datong University,Datong 037009,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2023年第20期2579-2583,2590,共6页
The Journal of Practical Medicine
基金
山西省基础研究计划(编号:202303021211326)。
