摘要
花期是玉米重要性状之一,解析玉米花期的遗传基础,挖掘玉米花期关键基因,对于选育广适玉米品种具有重要意义。在580份玉米自交系构成的自然群体中,3年种植测定散粉期、吐丝期和散粉吐丝间隔期等3个花期性状,利用分布全基因组的31,826个SNPs(single nucleotide polymorphisms)标记进行全基因组关联分析(genome wide association study,GWAS),结合自交系B73的14个不同发育阶段的转录组数据进行权重基因共表达网络分析(weighted gene co-expression network analysis,WGCNA),挑选与玉米开花相关的组织特异性模块和关键基因。GWAS在多环境(2个环境以上)下共定位标记14个,挖掘到潜在候选基因10个,WGCNA挖掘到花期潜在候选基因17个,2种方法共同挖掘到候选基因3个。Zm00001d052180编码一个MADS-box转录因子19,Zm00001d016814编码NAC转录因子133,Zm00001d048082编码MADS-box转录因子8,这些基因主要参与调节花序生长发育。研究结果为解析玉米花期遗传基础及分子机制提供参考。
The flowering time is one of the important traits in maize.It is of great significance to analyze the genetic basis and to mine the key core genes in flowering for maize varieties with wide adaptability.A natural population consisting of 580 maize inbred lines were planted for three years,to determine the three flowering traits(including days to anthesis,days to silking,and anthesis silking interval).Genome-wide association analysis was conducted using 31,826 SNPs distributed throughout the whole genome.Combined with transcriptome data of 14 different developmental stages of inbred line B73,weighted gene co-expression network analysis was performed to select tissue specific modules and key genes related to maize flowering time.A total of 14 SNPs for four flower traits under multiple environments and 10 potential candidate genes were mined by GWAS,WGCNA was used to mine 17 potential candidate genes in flowering time,three candidate genes were jointly mined by both approaches.Zm00001d052180 encodes a MADS box transcription factor 19,Zm00001d016814 encodes the NAC transcription factor 133,Zm00001d048082 encodes MADS box transcription factor 8,mainly involved in regulating inflorescence growth and development,which has certain research value and significance.These results provide a reference for the genetic basis and molecular mechanisms of flowering time related traits in maize.
作者
钱甫
张占琴
陈树宾
丁永福
桑志勤
李卫华
QIAN Fu;ZHANG Zhan-Qin;CHEN Shu-Bin;DING Yong-Fu;SANG Zhi-Qin;LI Wei-Hua(College of Agriculture,Shihezi University/Key Laboratory of Oasis Eco-Agriculture,Shihezi 832000,Xinjiang,China;Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,Xinjiang,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第12期3261-3276,共16页
Acta Agronomica Sinica
基金
兵团重点领域科技攻关项目(2019AB021)
兵团科技创新人才计划项目(2021CB038)
中国科学院“西部青年学者”项目资助。
