摘要
目的:探讨补肝健腰方对腰椎间盘突出大鼠模型血清代谢组学的影响。方法:将60只大鼠按随机数字表法,分为正常组12只、假手术组12只、造模组36只。造模组36只大鼠进行自身尾椎椎间盘移植法腰椎间盘突出模型(L5/6)造模处理,假手术组只暴露L5神经根,不给予自体尾椎椎间盘移植。对造模后进行神经功能评分及观察其行为学改变,造模成功后,将造模组分为模型组、中剂量组、高剂量组,每组12只,并进行中药干预。其中中、高剂量组分别给予补肝健腰方中、高剂量汤剂灌胃,正常组、模型组、假手术组,给予等剂量生理盐水灌胃。分别于干预后第1,2,3周采集大鼠腹主动脉血清进行代谢组学分析,回收包埋在L5神经根处的尾椎椎间盘标本,HE染色后用光学显微镜下对标本进行组织形态学观察。结果:通过自体尾椎椎间盘移植法成功造出腰椎间盘突出模型,血清中共筛选出多种代谢差异物,对筛选出的谷氨酸、乳酸进行分析,发现腰椎间盘突出大鼠模型血清中谷氨酸、乳酸发生了动态变化。造模后大鼠腰椎间盘组织在药物干预后第1,2,3周镜下观察均在呈现不同程度的形态学改变,补肝健腰方中、高剂量组均在干预后第3周镜下观察大鼠腰椎间盘突出组织较之前得到改善。结论:补肝健腰方通过对大鼠血清中谷氨酸、乳酸的影响,对腰椎间盘突出具有干预作用,可能是补肝健腰方治疗腰椎间盘突出症的机制之一。
Objective:To investigate the effect of bugan jianyao decoction on serum metabolomics of rats with lumbar disc herniation.Methods:60 rats were randomly divided into normal group(12 rats),sham operation group(12 rats)and model group(36 rats).In the model group,36 rats were treated with lumbar disc herniation model(L5/6)by autologous caudal intervertebral disc transplantation.In the sham operation group,only L5 nerve root was exposed,and autologous caudal intervertebral disc transplantation was not given.After the establishment of the model,the neurological function score and the behavioral changes were observed.After the establishment of the model,the model group was divided into model group,medium dose group and high dose group,with 12 rats in each group,and the intervention of traditional Chinese medicine was carried out.The medium and high dose groups were given the medium and high dose decoction of bugan jianyao decoction by gavage,and the normal group,model group and sham operation group were given the same dose of normal saline by gavage.At the 1st,2nd and 3rd week after intervention,the serum of rat abdominal aorta was collected for metabonomic analysis,and the specimens of caudal intervertebral disc embedded in L5 nerve root were recovered.After hematoxylin-eosin(HE)staining,the specimens were observed under optical microscope for histomorphology.Results:The lumbar intervertebral disc herniation model was successfully created by the rat autologous coccygeal intervertebral disc transplantation method.A variety of metabolic differences were screened in the serum,and the screened glutamic acid and lactic acid were analyzed.It was found that the serum glutamic acid and lactic acid changed dynamically in the rat model of lumbar disc herniation.After modeling,the lumbar intervertebral disc tissue of rats showed different degrees of morphological changes under the microscope at 1st,2nd and 3rd week after the drug intervention.It was observed that the herniated tissue of the lumbar intervertebral disc of the rat wa
作者
尹晨东
仇湘中
YIN Chendong;QIU Xiangzhong(Hunan College of Traditional Chinese Medicine,Zhuzhou 412012,Hunan China;Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine,Changsha 410006,China.)
出处
《中国中医骨伤科杂志》
CAS
2023年第9期7-14,共8页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家中医药管理局“全国名老中医药专家仇湘中传承工作室建设项目”(国中医药人教发[2016]42号)
湖南省研究生科研创新项目(CX20200776)
湖南中医药高等专科学校校级科研计划项目(XY2022.002)。
关键词
补肝健腰方
腰椎间盘突出大鼠模型
代谢组学
Bugan Jianyao decoction
rat model of lumbar intervertebral disc herniation
metabolomics
