摘要
背景:刮痧干预腰椎间盘突出症在临床得到广泛应用,利用代谢组学等分析技术对其作用机制进行研究。目的:使用气相色谱-质谱联用技术的代谢组学方法,探讨刮痧治疗腰椎间盘突出症大鼠尿液内源性代谢物的变化与其作用机制,并分析不同刮痧方案的疗效差异,以期规范标准化刮痧疗法,为临床应用提供参考和依据。方法:将48只雄性SD大鼠随机分为空白组、模型组、循经出痧组、穴位出痧组、循经不出痧组以及穴位不出痧组,各8只。选用自体髓核移植法复制腰椎间盘突出症大鼠模型,从造模第5天始,用不同刮痧方案干预,隔日1次,共9次。实验于2017年1月获得南京中医药大学实验动物伦理委员会审理批准。结果与结论:①同造模前相比,刮痧组及造模后的模型组机械痛阈值下降(P<0.01);②筛选后共得出15种潜在生物标志物和6条代谢通路。刮痧后3-(3-羟基苯基)丙酸、天冬氨酸和谷氨酰含量降低,甘氨酸、柠檬酸、环腺苷酸含量上升,通过调节丙氨酸,天冬氨酸和谷氨酸代谢通路以镇痛,甘氨酸,丝氨酸和苏氨酸代谢、乙醛酸和二羧酸代谢和三羧酸循环以抗炎;③循经出痧组调节代谢物效果优于其他3组,推测其为治疗最佳刮痧方案。
BACKGROUND:Scraping has been extensively applied in the treatment of lumbar disc herniation,and the underlying mechanism was explored by metabolomics.OBJECTIVE:To investigate the changes of endogenous metabolites in urine of rats with lumbar disc herniation and its mechanism of action by gas chromatography-mass spectrometry metabolomics method,and to analyze the difference in efficacy of different scraping schemes,in order to standardize the scraping therapy,and provide reference and basis for clinical application.METHODS:Forty-eight male Sprague-Dawley rats were randomly divided into blank,model and scraping groups(passing the sputum,acupuncture,sputum,and acupoint groups)(n=8 per group).The rat model of lumbar disc herniation was replicated by autologous nucleus pulposus transplantation.From the 5^th day of modeling,the scraping group was intervened with different scraping schemes,once every other day,totally nine times.The study was approved by the Experimental Animal Ethics Committee of Nanjing University of Traditional Chinese Medicine in January 2017.RESULTS AND CONCLUSION:(1)Compared with before modeling,the mechanical pain threshold in the scraping and the model groups after modeling was decreased(P<0.01).(2)A total of 15 potential biomarkers and 6 metabolic pathways were obtained after screening.After scraping,the content of 3-(3-hydroxyphenyl)propionic acid,aspartic acid and glutamic acid decreased,and the content of glycine,citric acid and cyclic adenosine increased,by adjusting alanine,aspartic acid and glutamine.The acid metabolism pathway was anti-inflammatory with analgesic,glycine,serine and threonine metabolism,glyoxylic acid and dicarboxylic acid metabolism,and a tricarboxylic acid cycle.(3)To conclude,the effect of regulating metabolites in the sputum group is better than that in the other three groups,which is presumed to be the best cure plan.
作者
丁欢
陈丽虹
陈宇婧
岳容兆
杨敏
徐桂华
Ding Huan;Chen Lihong;Chen Yujing;Yue Rongzhao;Yang Min;Xu Guihua(School of Nursing,Nanjing University of Traditional Chinese Medicine,Nanjing 210046,Jiangsu Province,China;Nanjing University of Traditional Chinese Medicine,Nanjing 210046,Jiangsu Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第23期3654-3659,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(81473791),项目负责人:徐桂华
江苏省研究生科研与实践创新计划项目(SJCX18_0444),项目负责人:陈丽虹~~
关键词
尿液代谢组学
刮痧
循经取穴
腰椎间盘突出
气相色谱-质谱
组织构建
代谢通路
国家自然科学基金
urine metabolomics
scraping
sputum
lumbar disc herniation
gas chromatography-mass spectrometry
tissue construction
metabolic pathways
the National Natural Science Foundation of China
