摘要
目的观察西红花苷对糖尿病肾病(diabetic nephropathy,DN)大鼠的作用,并探讨相关机制。方法将30只DN大鼠随机分为DN组、西红花苷组、西红花苷联合脂多糖(lipopolysaccharide,LPS)[Toll样受体4(toll like receptor 4,TLR4)/核转录因子κB(nuclear transcription factorκB,NF-κB)p65通路激活剂]组,各10只;另取10只大鼠,设为对照组。西红花苷联合LPS组灌胃西红花苷(40 mg·kg^(-1)),尾静脉注射LPS(0.4 mg·kg^(-1));西红花苷组灌胃西红花苷(40 mg·kg^(-1)),尾静脉注射等量生理盐水;对照组、DN组分别灌胃、尾静脉注射等量生理盐水。每日1次,干预2周。检测糖脂指标及肾功能指标;检测血清炎症因子水平;Western blotting检测肾组织TLR4、髓样分化因子88(myeloid differentiation factor 88,MyD88)、p-NF-κB p65、NF-κB p65蛋白的表达量。结果与DN组比较,西红花苷组血液空腹血糖(fasting plasma glucose,FPG)、空腹胰岛素(fasting serum lisulin,FINS)、三酰甘油(triglycerides,TG)和总胆固醇(triglycerides,TC)水平、24 h尿蛋白、微量白蛋白与尿肌酐比值(albumin/creatinine ratio,UACR)、血清肌酐(serum creatinine,Scr)和尿素氮(blood urea nitrogen,BUN)水平、血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)水平及肾组织TLR4、MyD88蛋白表达量及p-NF-κB p65/NF-κB p65均降低(P<0.05);与西红花苷组比较,西红花苷联合LPS组上述指标均升高(P<0.05)。结论西红花苷可改善糖脂代谢及肾功能,减轻炎症反应,从而抑制DN进展,推测其作用机制与抑制TLR4/NF-κB p65信号通路有关。
Objective To observe the inhibitory effect of crocin on the progression of diabetic nephropathy(DN)in diabetic rats,and to explore the related mechanism.Methods 30 DN rats were randomly divided into DN group,crocin group,crocin combined with lipopolysaccharide(LPS)[Toll-like receptor 4(TLR4)/nuclear transcription factorκB(NF-κB)p65 pathway activator]group,each with 10 rats.Another 10 healthy rats were selected as the control group.In the crocin combined with LPS group,crocin(40 mg·kg^(-1))was administered by gavage,and LPS(0.4 mg·kg^(-1))was injected into the tail vein.The crocin group was given intragastric administration of crocin(40 mg·kg^(-1)),and the same volume of normal saline was injected into the tail vein.The control group and the DN group were given the same volume of normal saline by gavage and tail vein injection respectively.The interventions were once a day for 2 weeks.Glycolipid indexes and renal function indexes were measured.Serum inflammatory factor levels were detected.Western blotting was used to detect the protein expressions of TLR4,myeloid cell differentiation factor 88(MyD88),p-NF-κB p65 and NF-κB p65 in renal tissue.Results Compared with the DN group,the blood fasting blood glucose(FPG),fasting insulin(FINS),triglyceride(TG),cholesterol(TC)levels,24-hour urine protein,microalbumin and urine creatinine ratios(UACR),serum creatinine(Scr),blood urea nitrogen(BUN)levels,serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)levels,renal tissue TLR4,MyD88 protein expression and p-NF-κB p65/NF-κB p65 were decreased in the crocin group(P<0.05).Compared with the crocin group,the above indexes were all increased in the crocin combined with LPS group(P<0.05).Conclusion Crocin can improve glucose and lipid metabolism and renal function,reduce inflammatory response,thereby inhibiting the progression of DN.The action is related to the inhibition of TLR4/NF-κB p65 signaling pathway.
作者
邹珊珊
邹珊
于小雪
马志超
ZOU Shanshan;ZOU Shan;YU Xiaoxue;MA Zhichao(Department of Drug Supply,the Affiliated Hospital of Chifeng College,Chifeng 024000,China;Department of Propaganda,Chifeng Industrial Vocational and Technical College,Chifeng 024000,China;Department of Medical Engineering,the Affiliated Hospital of Chifeng College,Chifeng 024000,China)
出处
《西北药学杂志》
CAS
2023年第6期83-88,共6页
Northwest Pharmaceutical Journal
基金
2022年赤峰市自然科学科研课题(编号:SZR2022135)。
