摘要
纤维素是植物细胞壁的主要成分,纤维素合成酶是纤维素合成过程中的重要酶类.通过生物信息学方法对芹菜纤维素合成酶(Apium graveliens cellulose synthases A,AgCESA)基因家族进行了全基因组水平的鉴定,共获得了11个AgCESA基因家族成员,将其分为5组(Ⅰ~Ⅴ);蛋白结构域分析显示所有AgCESA蛋白都具有纤维素合酶结构域和锌指结构域,并且在不同物种中也非常保守;AgCESA基因启动子区域均存在许多与光、激素和逆境响应相关的顺式作用元件,表明该家族基因可能参与植物生长发育和抗逆防御等多种生物学进程;对组织进行表达分析显示AgCESA基因在不同组织中表达水平有明显差异,在茎中表达量最高,叶次之,在根中表达量最低.研究结果明确了芹菜中的CESA编码基因,揭示了AgCESA基因在不同组织中的表达规律,为后续深入研究芹菜AgCESA基因功能奠定了重要的理论基础.
Cellulose is the main component of plant cell wall and cellulose synthetase is the key enzyme in the process of cellulose synthesis.In this study,the Apium graveliens CESA(AgCESA)gene family was identified at the whole-genome level using bioinformatics methods,and a total of 11 AgCESA gene family members were obtained and divided into five groupsⅠ~Ⅴ.Protein domain analysis showed that all AgCESA proteins had Cellulose_synt and zf-UDP conserved domains,which were quite conserved in different species.The promoter region of AgCESA genes contained a large number of light-responsive,hormone-responsive and stress-related cis-acting elements,suggesting that AgCESA genes might be involved in various biological processes including plant growth and environmental stress.Tissue expression analysis showed that the expression levels of AgCESA genes in different tissues were significantly different,with the highest expression in stems,followed by leaves,and the lowest expression in roots.The results from this study clarified the CESA-coding genes in celery,and revealed the expression patterns of AgCESAgenes,which laid an important theoretical foundation for further functional study of CESAgenes in celery.
作者
郭新月
张红梅
刘洪博
李艳玲
张晴
袁敏
GUO Xinyue;ZHANG Hongmei;LIU Hongbo;LI Yanling;ZHANG Qing;YUAN Min(College of Life Sciences,North China University of Science and Technology,Hebei Tangshan 063210,China)
出处
《河北师范大学学报（自然科学版）》
CAS
2023年第6期611-619,共9页
Journal of Hebei Normal University：Natural Science
基金
河北省自然科学基金(C2020209020)
河北省重点研发计划项目(20326513D)。
