摘要
目的:观察艾灸“命门”“关元”对健康大鼠免疫功能、肠道菌群的影响,探讨艾灸调节免疫功能的作用机制。方法:将20只8周龄SD大鼠随机分为青幼年空白组和青幼年艾灸组,每组10只;将20只8月龄SD大鼠随机分为中年空白组和中年艾灸组,每组10只。2个艾灸组大鼠予艾灸“命门”“关元”,每次15 min,每日1次,每周5次,共4个月;2个空白组正常饲养。干预结束后,计算大鼠胸腺、脾脏指数;采用HE染色法观察大鼠胸腺、脾脏组织形态;采用流式细胞术检测大鼠T淋巴细胞亚群CD_(4)^(+)、CD_(8)^(+)表达并计算CD_(4)^(+)/CD_(8)^(+)值;采用ELISA法检测大鼠血清肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)含量;采用16S rRNA高通量测序技术检测大鼠肠道菌群。分析肠道菌群相对丰度与血清TNF-α、IFN-γ、IL-6、IL-10、IL-17含量的相关性。结果:与青幼年空白组比较,青幼年艾灸组胸腺组织皮质面积增加,且淋巴细胞排列更紧密;与中年空白组比较,中年艾灸组大鼠胸腺指数增加(P<0.05),胸腺组织皮质面积增加。2个艾灸组和2个空白组脾脏指数比较差异无统计学意义(P>0.05)。2个艾灸组CD_(4)^(+)、CD_(8)^(+)表达及CD_(4)^(+)/CD_(8)^(+)值与同年龄段空白组比较差异无统计学意义(P>0.05)。与青幼年空白组比较,青幼年艾灸组IL-6含量升高(P<0.05);与中年空白组比较,中年艾灸组IL-10、IL-17含量降低(P<0.05)。与青幼年空白组比较,青幼年艾灸组Sobs指数、Ace指数、Chao指数升高(P<0.01,P<0.05),螺旋体门、密螺旋体属、苏黎世杆菌属、理研菌科RC9肠道群相对丰度升高(P<0.05),杜氏乳杆菌属相对丰度降低(P<0.05);与中年空白组比较,中年艾灸组大鼠螺旋体门、密螺旋体属、未分类的消化球菌属相对丰度升高(P<0.05),变形菌门、异杆菌属、粪杆菌属相对丰度降低(P<0.05)。嗜�
Objective To observe the effects of moxibustion at"Mingmen"(GV 4)and"Guanyuan"(CV 4)on immune function and intestinal flora in healthy rats,thereby investigating the underlying mechanism of moxibustion on immune function.Methods Twenty 8-week-old SD rats were randomly divided into a young blank group and a young moxibustion group,with 10 rats in each group.Similarly,twenty 8-month-old SD rats were randomly divided into a middle-aged blank group and a middle-aged moxibustion group,with 10 rats in each group.The rats in the two moxibustion groups received moxibustion at"Mingmen"(CV 4)and"Guanyuan"(CV 4),15 min per session,once daily,five times a week,for a total of four months.The rats in the two blank groups were fed under normal conditions.After the intervention,thymus and spleen indexes were calculated;the morphology of thymus and spleen tissues was observed using HE staining;the flow cytometry was used to detect the expression of CD_(4)^(+) and CD_(8)^(+) T lymphocytes and the CD_(4)^(+)/CD_(8)^(+),ratio was calculated;ELISA was used to measure the serum levels of tumor necrosis factor-alpha(TNF-α),interferon-gamma(IFN-γ),interleukin-6(IL-6),interleukin-10(IL-10),and interleukin-17(IL-17);16S rRNA high-throughput sequencing was used to analyze the intestinal flora.Additionally,the correlation between the relative abundance of intestinal flora and serum levels of TNF-α,IFN-γ,IL-6,IL-10 and IL-17 was analyzed.Results Compared with the young blank group,the young moxibustion group exhibited an increase in the cortical area of thymus tissue with tighter lymphocyte arrangement;compared with the middle-aged blank group,the middle-aged moxibustion group showed an increase in thymus index(P<0.05)and an increase in the cortical area of thymus tissue.There were no significant differences in spleen index between the 2 moxibustion groups and the 2 blank groups(P>0.05).There were no significant differences in the expression of CD_(4)^(+),CD_(8)^(+),and CD_(4)^(+)/CD_(8)^(+);ratio between the 2 moxibustion groups and the
作者
张军花
单思
王沐晨
邹泽晗
刘红宁
ZHANG Jun-hua;SHAN Si;WANG Mu-chen;ZOU Ze-han;LIU Hong-ning(Research Center for Differentiation and Development of Basic Theory of Traditional Chinese Medicine,Jiangxi University of CM/Jiangxi Province Key Laboratory of Traditional Chinese Medicine Etiopathogenisis,Nanchang 330008,China)
出处
《中国针灸》
CAS
CSCD
北大核心
2023年第10期1157-1164,共8页
Chinese Acupuncture & Moxibustion
基金
江西民族传统药现代科技与产业发展协同创新中心项目:赣高字[2013]109号。
关键词
艾灸
免疫功能
肠道菌群
淋巴细胞
moxibustion
immune function
intestinal flora
lymphocytes
