摘要
目的探讨长链非编码RNA(lncRNA)ANRIL、lncRNA CAI2在脑梗死患者的血清表达及两者对脑梗死的诊断价值。方法采用实时荧光定量PCR技术及酶联免疫吸附测定法检测脑梗死患者与健康志愿者(对照组)血清lncRNA ANRIL、lncRNA CAI2及炎症因子水平,比较两组差异;采用ROC曲线评估lncRNA ANRIL、lncRNA CAI2及其联合分析对脑梗死的诊断价值;采用Spearman相关性分析评价lncRNA ANRIL、lncRNA CAI2与血清炎症因子的相关性。结果与对照组相比,脑梗死患者血清炎症因子超敏C反应蛋白(hs-CRP)、白细胞介素(IL)-6、IL-8、肿瘤坏死因子α(TNF-α)水平显著上调(t=25.485、49.691、47.328、41.763,P<0.05),且lncRNA ANRIL、lncRNA CAI2也显著上调(t=-9.264、-9.790,P<0.05)。lncRNA ANRIL、lncRNA CAI2及其联合对脑梗死诊断的ROC曲线AUC分别为0.801、0.825、0.901。lncRNA ANRIL(r=0.412、0.438、0.493、0.481)、lncRNA CAI2(r=0.439、0.422、0.477、0.441)与hs-CRP、IL-6、IL-8、TNF-α均呈显著正相关(P<0.01)。结论lncRNA ANRIL、lncRNA CAI2在脑梗死患者血清高表达,两者及两者联合均对脑梗死具有一定诊断价值。
Objective To investigate the serum expression of long non-coding(lnc)RNA ANRIL and lncRNA CAI2 in patients with cerebral infarction and its value in the diagnosis of cerebral infarction.Methods The levels of lncRNA ANRIL,lncRNA CAI2 and inflammatory factors in the serum of cerebral infarction patients and healthy volunteers(control group)were detected by real-time quantitative PCR and enzyme-linked immunosorbent assay,and the indicators in the two groups were compared.ROC curve was used to evaluate the value of lncRNA ANRIL,lncRNA CAI2 and their combined analysis in the diagnosis of cerebral infarction.Spearman correlation analysis was used to assess the correlation between lncRNA ANRIL,lncRNA CAI2 and serum inflammatory factors.Results Compared with the control group,serum inflammatory factors,including high sensitivity C-reactive protein(hs-CRP),interleukin-6(IL-6),IL-8 and tumor necrosis factor(TNF-α)were significantly up-regulated in cerebral infarction patients(t=25.48549.691,47.328,41.763;P<0.05),the levels of lncRNA ANRIL and lncRNA CAI2 were also significantly up-regulated(t=-9.264,-9.790;P<0.05).The AUC of ROC for lncRNA ANRIL,lncRNA CAI2 and their combination in diagnosing cerebral infarction were 0.801,0.825 and 0.901,respectively.lncRNA ANRIL(r=0.412,0.438,0.493,0.481)and lncRNA CAI2(r=0.439,0.422,0.477,0.441)were significantly positively correlated with hs-CRP,IL-6,IL-8 and TNF-α(P<0.01).Conclusion lncRNA ANRIL and lncRNA CAI2 are highly expressed in the serum of patients with cerebral infarction.Both lncRNA ANRIL and lncRNA CAI2,as well as their combination have certain diagnostic value for cerebral infarction.
作者
刘晓亮
郑小军
王英
刘娟
Liu Xiaoliang;Zheng Xiaojun;Wang Ying;Liu Juan(Department of Neurology,People’s Hospital of Baoji,Baoji 721000,China)
出处
《心脑血管病防治》
2023年第6期5-8,共4页
CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT
关键词
长链非编码RNA
脑梗死
血清炎症因子
Long non-coding RNA
Cerebral infarction
Serum inflammatory factors
