摘要
目的探讨银屑病血清与五联因子(M5)对角质形成细胞炎症和增殖影响的差异性。方法分别用健康人、银屑病患者血清和M5因子对人类永生化角质形成细胞(Human immortalized keratinocyte cell line,HaCaT)进行培养,通过CCK-8监测细胞的增殖情况,并采用RT-qPCR检测各组HaCaT细胞中炎症因子白介素1β/8/21/23(IL-1β/8/21/23)与增殖标志物角蛋白6/16(K6/K16)和细胞相关抗原67(Ki67)的mRNA表达水平。结果健康人、银屑病血清和M5因子均能有效促进HaCaT细胞的生长,且银屑病血清和M5因子对细胞生长的促进作用强于健康人血清。与M5因子相似,银屑病患者血清显著促进细胞中炎症因子IL-1β、IL-8、IL-23和增殖标志物K6、Ki67的表达;与M5因子不同的是,银屑病患者血清对炎症因子IL-21的促进作用增强,而对增殖标志物K16的促进作用减弱。结论银屑病患者血清微环境对角质形成细胞增殖和炎症反应的促进作用高于健康人,且与M5因子相似,可以用于构建银屑病角质形成细胞模型。
Objective To explore the differences in the effects of psoriatic serum and a mixture of five proinflammatory cytokines on keratinocyte inflammation and proliferation.Methods The human immortalized keratinocyte cell line(HaCaT cells)was used to cultivate with serum of healthy human serum,psoriasis patients and M5 factors,and the cell proliferation was monitored by CCK-8.RT-qPCR was used to detect the mRNA expression levels of inflammatory factors interleukin 1β/8/21/23(IL-1β/8/21/23),proliferation markers keratin 6/16(K6/K16)and nucleus related antigen 67(Ki67)in HaCaT cells in each group.Results Healthy human serum,psoriasis serum and M5 factors could effectively promote the growth of HaCaT cells,and psoriasis serum and M5 factors could promote cell growth more than healthy human serum.Similar to M5 factors,serum from patients with psoriasis significantly promoted the mRNA levels of inflammatory factors IL-1β,IL-8,IL-23 and proliferation markers K6 and Ki67 in cells.Different from M5 factors,the serum of psoriasis patients had enhanced the mRNA level of inflammatory factor IL-21,but weakened the promotion of proliferation marker K16.Conclusion The serum microenvironment of patients with psoriasis can promote the proliferation and inflammatory response of keratinocytes higher than that of healthy people,and is similar to M5 factors,which can be used to build a keratinocyte model of psoriasis.
作者
王泽洪
李俊琴
邹朋
李新华
Wang Zehong;Li Junqin;Zou Peng;Li Xinhua(Dept of Microbiology and Immunology,School of Basic Medical Sciences,Shanxi Medical University,Jinzhong 030619;Dept of Dermatology,Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis,Taiyuan Central Hospital of Shanxi Medical University,Taiyuan 030009)
出处
《安徽医科大学学报》
CAS
北大核心
2023年第9期1488-1492,共5页
Acta Universitatis Medicinalis Anhui
基金
山西省重点医学攻关专项(编号:2020XM20)。
关键词
银屑病
角质形成细胞
炎症
增殖
psoriasis
keratinocytes
inflammation
proliferation
