摘要
鉴定了紫连膏的主要化学成分,并研究紫连膏对咪喹莫特银屑病大鼠治疗作用.采用HPLC高效液相色谱法分析鉴定紫连膏甲醇提取物主要成分;SRB法检测紫连膏甲醇提取物对永生化角质形成细胞HaCaT的增殖影响;Annexin V-FITC/PI双染法检测紫连膏甲醇提取物对HaCaT细胞凋亡的作用;建立Wistar大鼠银屑病模型,研究紫连膏对大鼠银屑病病理及相关细胞因子的影响.研究发现紫连膏含有乙酰氧基异戊酰阿卡宁、龙胆苦苷、胡黄连苷;紫连膏甲醇提取物对HaCaT细胞有一定增殖抑制作用IC_(50)=172.55和300μg/mL的紫连膏甲醇提取物对HaCaT细胞有极显著的促凋亡作用(p<0.01);经过紫连膏治疗后,紫连膏高剂量组显著减少了银屑病大鼠皮肤的鳞屑、红斑、厚度(p<0.05);造模皮肤组织中IL-10的mRNA表达量上升,IL-17的mRNA表达量下降(p<0.05);降低了银屑病大鼠血清中TNF-α的含量,增加了皮肤中IL-10的含量(p<0.05).研究表明紫连膏中含有乙酰氧基异戊酰阿卡宁、龙胆苦苷、胡黄连苷;紫连膏可以通过诱导角质细胞凋亡,调节IL-10、IL-17、TNF-α来治疗银屑病.
This study analyzed and identified the main chemical components of Zilian ointment,and studied the therapeutic effect of Zilian ointment on imiquimod psoriasis rats.HPLC was used to identify the main components of methanolic extract of Zilian ointment;The effect of methanol extract of Zilian ointment on the proliferation of immortalized keratinocytes HaCaT was checked by SRB method;Annexin V-FITC/PI double-staining method was used to detect the effect of methanolic extract of Zilian ointment on the cell apoptosis of HaCaT cells;Imiquimod-induced psoriasis-like dermatitis in rat was established to study the effects of Zilian ointment on the pathology and related cytokines of psoriasis in rats.It was found that Zilian ointment contained Acetoxyisovalerylalkannin,Gentiopicroside and Picroside;the methanolic extract of Zilian ointment had a proliferation inhibitory effect on HaCaT cells IC_(50)=172.55μg/mL;the methanolic extract of Zilian ointment at 300μg/mL had a significant pro-apoptotic effect on HaCaT cells(p<0.01);after treatment with Zilian ointment,the high dose of Zilian ointment group significantly reduced the scaling,erythema,and thickness of the skin of psoriatic rats(p<0.05);The expression of IL-10 in the skin tissue of rats in the imiquimod group increased and the expression of IL-17 decreased(p<0.05);Zilian ointment decreased the content of TNF-αin the serum and increased the content of IL-10 in the skin of rats with psoriasis(p<0.05).Research shows that Zilian ointment contains Acetoxyisovalerylalkannin,Gentiopicroside and Picroside;it can treat psoriasis by inducing apoptosis in keratinocytes and regulating IL-10,IL-17 and TNF-α.
作者
李元策
孙玉梅
王艳雯
杨雪松
尹俊林
叶建州
LI Yuan-che;SUN Yu-mei;WANG Yan-wen;YANG Xue-song;YIN Jun-lin;YE Jian-zhou(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education,School of Ethnic Medicine,Yunnan Minzu University,Kunming 650500,China;The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Specialized Hospital of Dermatology,Kunming 500021,China)
出处
《云南民族大学学报（自然科学版）》
CAS
2023年第4期428-435,共8页
Journal of Yunnan Minzu University:Natural Sciences Edition
基金
国家自然科学基金(21768005)
云南省重大科技专项项目(2017ZF011)
云南省高校重点实验室建设计划.
关键词
紫连膏
银屑病
卡泊三醇
Zilian ointment
psoriasis
calcipotriol
