摘要
目的探讨妊娠期高血压疾病(HDP)患者长链非编码RNA FAM99A(LncRNA FAM99A)的表达及其临床意义。方法选取183例HDP患者为HDP组和50例健康孕妇为对照组,检测所有研究对象血清LncRNA FAM99A及白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及超敏C反应蛋白(hs-CRP)水平。根据HDP患者病情严重程度分为妊娠期高血压(GH)48例、轻度子痫前期(MPE)74例和重度子痫前期(SPE)61例,根据HDP患者出现不良妊娠结局情况分为不良妊娠结局组68例和妊娠结局良好组115例。绘制受试者工作特征(ROC)曲线分析血清LncRNA FAM99A表达水平预测SPE及不良妊娠结局的价值,分析HDP患者血清LncRNA FAM99A表达水平与IL-6、TNF-α及hs-CRP的相关性。结果HDP组血清LncRNA FAM99A表达水平(0.94±0.26 vs.2.37±0.85)明显低于对照组(P<0.001),而血清IL-6[(158.24±16.73)pg/mL vs.(91.28±8.16)pg/mL]、TNF-α[(20.96±3.27)pg/mL vs.(8.40±1.25)pg/mL]及hs-CRP[(9.05±2.16)mg/L vs.(2.71±0.28)mg/L]水平均明显高于对照组(P<0.001)。随着HDP患者病情程度加重,血清LncRNA FAM99A表达水平逐渐降低(SPE组<MPE组<GH组),而血清IL-6、TNF-α及hs-CRP水平逐渐升高(SPE组>MPE组>GH组)。不良妊娠结局组血清LncRNA FAM99A表达水平(0.19±0.03 vs.1.35±0.46)明显低于妊娠结局良好组(P<0.001),而血清IL-6[(210.24±25.13)pg/mL vs.(136.20±15.37)pg/mL]、TNF-α[(34.72±7.05)pg/mL vs.(17.80±2.64)pg/mL]及hs-CRP[(16.30±5.17)mg/L vs.(7.95±2.08)mg/L]水平均明显高于妊娠结局良好组(P<0.001)。ROC曲线显示,LncRNA FAM99A≤0.65预测SPE发生的曲线下面积(AUC)最大(0.877,95%CI:0.814~0.936),敏感度为87.4%,特异度为85.0%;LncRNA FAM99A≤0.48预测HDP患者不良妊娠结局的AUC最大(0.870,95%CI:0.808~0.930),敏感度为85.2%,特异度为85.6%。相关分析显示,HDP患者血清LncRNA FAM99A表达水平与IL-6(r=-0.826,P<0.001)、TNF-α(r=-0.803,P<0.001)及hs-CRP(r=-0.715,P<0.001)均呈负相关。结论LncRNA FAM99A在HDP患者中呈低表达,与疾�
Objective To investigate the expression of long noncoding RNA FAM99A(LncRNA FAM99A)in patients with hypertensive disorder complicating pregnancy(HDP)and its clinical significance.Methods The serum levels of LncRNA FAM99A,interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and hypersensitive C-reactive protein(hs-CRP)were detected in 183 HDP patients as HDP group and 50 healthy pregnant women as control group.HDP patients were divided into 48 cases of gestational hypertension(GH),74 cases of mild preeclampsia(MPE)and 61 cases of severe preeclampsia(SPE)according to the severity of their condition.According to the adverse pregnancy outcome of HDP patients,they were divided into 68 cases of adverse pregnancy outcome group and 115 cases of good pregnancy outcome group.ROC curve was applied to analyze the value of serum LncRNA FAM99A expression level in predicting SPE and adverse pregnancy outcomes.The relationship between serum LncRNA FAM99A expression level and IL-6,TNF-αand hs-CRP in HDP patients were analyzed.Results The level of serum LncRNA FAM99A expression(0.94±0.26 vs.2.37±0.85)in HDP group was significantly lower than that in control group(P<0.001),while serum IL-6[(158.24±16.73)pg/mL vs.(91.28±8.16)pg/mL],TNF-α[(20.96±3.27)pg/mL vs.(8.40±1.25)pg/mL]and hs-CRP[(9.05±2.16)mg/L vs.(2.71±0.28)mg/L]were significantly higher than those in the control group(P<0.001).With the aggravation of HDP patients′condition,the level of serum LncRNA FAM99A expression gradually decreased(SPE group<MPE group<GH group),while serum IL-6,TNF-αand hs-CRP level increased gradually(SPE group>MPE group>GH group).The level of serum LncRNA FAM99A expression in the poor pregnancy outcome group(0.19±0.03 vs.1.35±0.46)was significantly lower than that in the good pregnancy outcome group(P<0.001),while the level of serum IL-6[(210.24±25.13)pg/mL vs.(136.20±15.37)pg/mL],TNF-α[(34.72±7.05)pg/mL vs.(17.80±2.64)pg/mL]and hs-CRP[(16.30±5.17)vs.(7.95±2.08)mg/L]were significantly higher than those in the group with good pregn
作者
邢维珍
王佳
宁静
符毓珍
XING Wei-zhen;WANG Jia;NING Jing;FU Yu-zhen(Department of Gynaecology and Obstetrics,Sanya Women and Children′s Hospital Managed by Shanghai Children′s Medical Center,Sanya 572000,Hainan,China)
出处
《广东医学》
CAS
2023年第8期985-990,共6页
Guangdong Medical Journal
基金
海南省省级临床医学中心建设项目[琼卫医函(2021)75号]。
