摘要
以总三萜提取率为评价指标,利用超声波提取技术结合单因素试验和响应面试验优化连翘(Forsythia suspensa(Thunb.)Vahl)叶总三萜的最佳提取工艺;并利用6种大孔树脂进行进一步纯化,再采用CCK-8法检测连翘叶总三萜对人乳腺癌MCF-7、MDA-MB-231细胞,肝癌HepG2、SNU-739细胞,结肠癌HT29、HCT116细胞的增殖抑制作用。结果表明,连翘叶总三萜最优提取工艺为乙醇浓度85%、料液比1∶40 g/mL、提取时间35 min、提取温度60℃,在此条件下连翘叶总三萜的提取率可达14.13%;D101型树脂为最佳树脂,经纯化的连翘叶总三萜的含量可达72.89%,连翘叶总三萜对MCF-7、MDAMB-231、HepG2、SNU-739、HT29和HCT116细胞的半数抑制浓度(IC50)分别为(6.29±0.34)、(9.51±0.71)、(12.05±1.23)、(9.91±0.94)、(22.82±0.65)、(23.45±1.01)mg/L。优化后的连翘叶总三萜提取工艺稳定简单,且其纯化后具有较强的抗肿瘤活性。
Taking the extraction rate of total triterpenes as the evaluation indicator,the optimum extraction technology of total triterpenes from leaves of Forsythia suspensa was optimized by ultrasonic extraction technology combined with single factor test and response surface test.Six kinds of macroporous resins were used for further purification,and CCK-8 method was used to detect the proliferation inhibitory effect of total triterpenes of Forsythia suspensa leaves on the human breast cancer MCF-7 and MDA-MB-231 cells,liver cancer HepG2 and SNU-739 cells,colon cancer HT29 and HCT116 cells.The results showed that the optimum extraction condition of total triterpenes from Forsythia suspensa leaves was ethanol concentration of 85%,ratio of material to liquid of 1∶40 g/mL,extraction time of 35 min,and extraction temperature of 60℃.Under this condition,the extraction rate of total triterpenes from Forsythia suspensa leaves could reach 14.13%.D101 resin was the best resin,and the content of total triterpenes from Forsythia suspensa leaves could reach 72.89%.The 50%inhibitory concentration(IC50)of total triterpenes from Forsythia suspensa leaves to MCF-7,MDA-MB-231,HepG2,SNU-739,HT29 and HCT116 cells was(6.29±0.34),(9.51±0.71),(12.05±1.23),(9.91±0.94),(22.82±0.65),and(23.45±1.01)mg/L,respectively.The optimized extraction process for total triterpenes from Forsythia suspensa leaves was stable and simple,and it had strong anti-tumor activity after purification.
作者
王学方
陈玲
归荣
宁二娟
王伟
王学兵
李晓
WANG Xue-fang;CHEN Ling;GUI Rong;NING Er-juan;WANG Wei;WANG Xue-bing;LI Xiao(Henan Natural Products Biotechnology Co.,Ltd.,Zhengzhou 450002,China;College of Animal Medicine,Henan Agricultural University,Zhengzhou 450000,China)
出处
《湖北农业科学》
2023年第9期113-118,174,共7页
Hubei Agricultural Sciences
基金
河南省科技攻关项目(222102310543)
中央引导地方科技发展专项基金项目(211213004)。
