摘要
为优化见血青[Liparis nervosa(Thunb.ex A.Murray)Lindl.]的酚类成分提取工艺,以酚类成分得率为指标,通过单因素试验研究超声功率、乙醇浓度、料液比和提取时间4个因素对见血青酚类成分得率的影响;并采用Box-Behnken试验设计进行响应面分析,确定提取见血青酚类成分的最佳工艺条件。结果显示,超声辅助提取见血青酚类成分的最佳提取工艺为以料液比1∶72(g∶mL)加入浓度72%的乙醇,用350 W超声辅助提取54 min,见血青酚类成分得率可达(14.31±1.26)mg·g^(-1)。与加热回流和浸渍这2种传统提取工艺相比,超声辅助提取具有耗能少、耗时短和酚类成分得率高等优点,有一定的应用价值。
To optimize the phenol extraction process of Liparis nervosa(Thunb.ex A.Murray)Lindl..The effects of four factors,namely,ultrasonic power,ethanol concentration,material-to-liquid ratio and extraction time,on the phenol yield of L.nervosa were investigated by single-factor test.The Box-Behnken experimental design was used for response surface analysis to determine the best technological conditions for the extraction of phenols from L.nervosa.The results showed that the best extraction process for the extraction of phenols was as follows:The phenol of L.nervosa were extracted with the aid of ultrasound for 54 min at a ratio of 1:72(g:mL)with the addition of 72% ethanol.The yield of phenol of L.nervosa was up to(14.31±1.26)mg·g^(-1).Compared with two traditional extraction methods of condensation reflux and maceration,ultrasound-assisted extraction has the advantages of less energy consumption,shorter time consumption and higher phenol yield,which has certain application value.
作者
迪明博
王润坤
邹美佳
薛诗意
刘量
DI Mingbo;WANG Runkun;ZOU Meijia;XUE Shiyi;LIU Liang(Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases/College of Medicine(Institute of Translational Medicine),Yangzhou University,Yangzhou 225009,China;Pharmacy Department of Xuyi People's Hospital,Xuyi 211700,China;Jiangsu Lianyungang Higher Vocational Technical School of Chinese Medicine,Lianyungang 222007,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2023年第4期68-75,共8页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省高校“青蓝工程”项目(20200104)
扬州大学“高端人才支持计划”项目(20190223)
扬州大学“青蓝工程”项目(20180210)。
关键词
见血青
酚类成分
超声波辅助提取
响应面法
传统提取工艺
Liparis nervosa(Thunb.ex A.Murray)Lindl.
phenols
ultrasound-assisted extraction process
response surface methodology
conventional extraction process
