摘要
目的:基于高效液相色谱法(HPLC)及相态拆分工艺探讨微乳对泽泻汤提取液不同相态中指标成分分布的影响。方法:采用粒径仪和透射电镜对空白微乳、泽泻汤水提液和泽泻汤微乳提取液中胶体粒子进行表征;采用高速离心法及透析法建立相态拆分工艺,并依据该工艺,将泽泻汤水提液和泽泻汤微乳提取液分别拆分为真溶液相态,胶体相态和沉淀相态;采用HPLC测定不同相态中白术内酯Ⅲ、白术内酯Ⅱ、23-乙酰泽泻醇C、泽泻醇A、泽泻醇B和23-乙酰泽泻醇B共6种指标成分的含量,流动相水(A)-乙腈(B)梯度洗脱(0~5 min,40%~43%B;5~20 min,43%~45%B;20~45 min,45%~60%B;45~75 min,60%~80%B);采用饱和溶解度方法测定泽泻汤指标成分在水和微乳溶剂中的溶解度。结果:泽泻汤水提液、微乳提取液和空白微乳中的胶体粒子形态均呈圆球形,胶体粒子的粒径、多分散指数(PDI)和Zeta电位大小排序均为水提液>微乳提取液>空白微乳。相态拆分结果显示,透析2.5 h即可将胶体相态和真溶液相态拆分完全,经检验该工艺稳定可行。与泽泻汤水提液比较,以微乳作为提取溶剂可以提高白术内酯Ⅲ、23-乙酰泽泻醇C、白术内酯Ⅱ、泽泻醇A、泽泻醇B和23-乙酰泽泻醇B在胶体相态内的含量约3.75、6.82、35.47、10.66、35.41、27.75倍,并可提高后5种成分的提取效率2.03、1.15、1.70、6.43、5.53倍。溶解度试验显示,微乳能提高白术内酯Ⅱ、泽泻醇A、泽泻醇B和23-乙酰泽泻醇B的溶解度,但对白术内酯Ⅲ和23-乙酰泽泻醇C的溶解度影响较小。结论:微乳可不同程度提高泽泻汤指标成分的提取效率并增加指标成分在胶体相态中的分布,为微乳作为中药提取溶剂的可行性提供参考。
Objective:To investigate the effect of microemulsion on the distribution of index components in different phases of Zexietang extract based on high performance liquid chromatography(HPLC)and phase separation process.Method:Particle size meter and transmission electron microscope were used to characterize the colloidal particles in blank microemulsion,aqueous extract of Zexietang and microemulsion extract of Zexietang.The phase separation process was established by high-speed centrifugation and dialysis,and based on this process,the aqueous extract and microemulsion extract of Zexietang were separated into the true solution phase,the colloidal phase and the precipitation phase,respectively.The contents of six components,including atractylenolideⅢ,atractylenolideⅡ,23-acetyl alisol C,alisol A,alisol B and alisol B 23-acetate,were determined by HPLC with the mobile phase of water(A)-acetonitrile(B)for gradient elution(0-5 min,40%-43%B;5-20 min,43%-45%B;20-45 min.45%-60%B;45-75 min,60%-80%B).The solubility of the index components in water and microemulsion was determined by saturation solubility method.Result:The colloidal particles in the aqueous extract,microemulsion extract and blank microemulsion were all spherical,and the particle size,polydispersity index(PDI)and Zeta potential of the colloidal particles were in the order of aqueous extract>microemulsion extract>blank microemulsion.The results of phase separation showed that the colloidal phase and the true solution phase could be completely separated by dialysis for 2.5 h,and the phase separation process was tested to be stable and feasible.Compared with the aqueous extract of Zexietang,the use of microemulsion as an extraction solvent could increase the contents of atractylenolideⅢ,23-acetyl alisol C,atractylenolideⅡ,alisol A,alisol B and alisol B 23-acetate by 3.75,6.82,35.47,10.66,35.41,27.75-fold,and could increase the extraction efficiencies of the latter five constituents by 2.03,1.15,1.70,6.43,5.53 times.The solubility test showed that the microe
作者
王艳静
欧则民
严林
张瑶
王梓丞
程怡
仝燕
刘德文
王锦玉
WANG Yanjing;OU Zemin;YAN Lin;ZHANG Yao;WANG Zicheng;CHENG Yi;TONG Yan;LIU Dewen;WANG Jinyu(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Beijing City University,Beijing 100089,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2023年第20期134-140,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国中医科学院科技创新工程项目(CI2021A04303)。
关键词
泽泻汤
微乳
相态拆分工艺
含量测定
胶体粒子
溶解度
提取溶剂
Zexietang
microemulsion
phase separation process
content determination
colloidal particles
solubility
extraction solvent
