摘要
沙门氏菌是一种革兰氏阴性短杆菌,血清型众多,可引起各种动物的细菌感染。为探究鸭肠炎沙门氏菌的致病性差异,本试验从山东省三个地区采集50份疑似沙门氏菌感染样品,通过细菌分离及PCR扩增目的基因进行鉴定,后通过动物回归试验研究此次分离的沙门氏菌在雏鸭中产生的影响,并通过PCR扩增及Blast比对分析对所分离到的沙门氏菌进行分型。结果显示,从山东省三个地区采集的50份样品中共分离鉴定出5株沙门氏菌;动物回归试验结果显示:5组沙门氏菌中,第5组相较其他组别致死率最高,致死率高达87.5%,其次为2组、4组、3组、1组;通过Blast比对分析,确定分离到的5株菌均为肠炎型沙门氏菌。本试验为山东地区鸭肠炎沙门氏菌流行情况及致病性差异提供了部分理论依据和数据支持。
In order to explore the pathogenic differences of Salmonella enterica in ducks,50 samples of S.enterica were collected from different areas of Shandong province.Five strains of S.enterica were successfully isolated through bacterial isolation and PCR identification.Pathogenicity tests were conducted to investigate the clinical symptoms and pathological changes caused by the isolated strains in the process of duck farming to determine the different pathogenicity.The results showed that among the five groups of S.enterica,the second and fifth groups had the highest pathogenicity rate and the strongest mortality rate compared to other groups.This experiment provides partial theoretical basis and data support to analysis the prevalence and pathogenic differences of S.enterica in duck of Shandong province.
作者
魏可欣
石锐琪
林仲寅
李世恒
李继桐
李琦
吕俊峰
WEI Kexin;SHI Ruiqi;LIN Zhongyin;LI Shiheng;LI Jitong;LI Qi;LV Junfeng(Poultry Institute,Shandong Academy of Agricultural Science,Jinan 250100,China)
出处
《家禽科学》
2023年第10期54-58,I0011,共6页
Poultry Science
基金
山东省农业科学院农业科技创新工程人才任务“家禽高效智慧健康养殖关键技术研究”(CXGC2023F11)。
关键词
鸭肠炎沙门氏菌
分离鉴定
致病性试验
Duck Salmonella enteritis
Isolation and identification
Pathogenicity
