摘要
目的探究姜黄素对慢性颞叶内侧癫痫大鼠脑脊液和血浆中基质金属蛋白酶(MMP)-9水平及血脑屏障通透性的影响。方法本研究于2021年2月至2023年2月在广州医科大学附属脑科医院开展。32只大鼠(6~8周,雌性,体质量160~180 g)中成功构建颞叶内侧癫痫模型大鼠17只,将17只成功建模的慢性颞叶内侧癫痫大鼠随机分到姜黄素组(9只)和生理盐水组(8只)。姜黄素组大鼠予以腹腔注射姜黄素(100 mg/kg)干预,生理盐水组大鼠予以腹腔注射生理盐水。分别检测两组大鼠干预前后脑脊液和血浆中白蛋白及MMP-9水平。采用t检验、单因素方差分析、事后多重比较。结果姜黄素组大鼠白蛋白商(脑脊液白蛋白/血浆白蛋白比值)从干预前的(14.443±1.700)下降至干预后的(12.822±1.450)(P<0.05),脑脊液MMP-9水平从干预前的(43.753±3.787)µg/L下降至干预后的(39.507±4.015)µg/L(P<0.05);而生理盐水组脑脊液和血浆中白蛋白水平、白蛋白商及MMP-9水平干预前后均无显著变化(均P>0.05)。结论姜黄素可能通过改变脑脊液中MMP-9水平来改变慢性颞叶内侧癫痫大鼠血脑屏障通透性,进而对受损的血脑屏障起到一定的修复作用。
Objective To investigate the effects of curcumin on the levels of matrix metalloproteinase(MMP)-9 in cerebrospinal fluid(CSF)and plasma and the permeability of blood-brain barrier in rats with chronic medial temporal lobe epilepsy.Methods The study was carried out in the Affiliated Brain Hospital of Guangzhou Medical University from February 2021 to February 2023.Among 32 rats(6-8 weeks,female,body mass 160-180 g),17 rats were successfully constructed with the model of medial temporal lobe epilepsy.Seventeen rats with chronic medial temporal lobe epilepsy were randomly divided into a curcumin treatment group(n=9)and a normal saline treatment group(n=8).The rats in the curcumin treatment group were intraperitoneally injected with curcumin(100 mg/kg),while the rats in the normal saline treatment group were intraperitoneally injected with normal saline.The levels of albumin and MMP-9 in CSF and plasma were detected before and after intervention.t test,one-way ANOVA,and multiple comparisons after ANOVA were used.Results The albumin quotient(ratio of CSF albumin/plasma albumin)of the rats in the curcumin treatment group decreased from(14.443±1.700)before intervention to(12.822±1.450)after intervention(P<0.05),and the level of MMP-9 in CSF decreased from(43.753±3.787)μg/L before intervention to(39.507±4.015)μg/L after intervention(P<0.05).There were no significant changes in the albumin and MMP-9 levels in CSF and plasma and the albumin quotient in the normal saline treatment group before and after intervention(all P>0.05).Conclusion Curcumin might change the permeability of blood-brain barrier in rats with chronic medial temporal lobe epilepsy by changing the level of MMP-9 in CSF,and play a certain role in repairing the damaged blood-brain barrier.
作者
郭彩凤
谭燕
张岳峰
周华荣
陈国华
方雅秀
蓝晓凤
Guo Caifeng;Tan Yan;Zhang Yuefeng;Zhou Huarong;Chen Guohua;Fang Yaxiu;Lan Xiaofeng(Department of Neurology,The Affiliated Brain Hospital of Guangzhou Medical University,Guangzhou510370,China)
出处
《国际医药卫生导报》
2023年第19期2771-2775,共5页
International Medicine and Health Guidance News
基金
广东省自然科学基金项目(2018A030313821)
广州市中医药和中西医结合科技项目(20192A010012、20232A010013)
广州市卫生健康科技项目(20221A010030)。
关键词
姜黄素
血脑屏障
MMP-9
慢性颞叶内侧癫痫
大鼠
动物实验
Curcumin
Blood-brain barrier
MMP-9
Chronic medial temporal lobe epilepsy
Rats
Animal experiment
