摘要
目的:探讨姜黄素对酒精中毒幼鼠学习、记忆能力的影响及其机制。方法:将雄性3周龄SD大鼠(50~70 g)按简单随机抽样的方法分成酒精组、对照组、治疗组、NaOH组,每组8只。酒精组用52%乙醇溶液灌胃[10 mL/(kg·d)]制作酒精中毒模型;对照组用等量生理盐水灌胃,余同酒精组;治疗组在酒精组基础上加用姜黄素NaOH溶液[100 mg/(kg·d)]进行腹腔注射;NaOH组仅用等体积、同浓度的NaOH进行腹腔注射。7 d后采用Morris水迷宫观察每组幼鼠的学习、记忆功能;13 d后采用免疫组化观察大脑海马冠状切面切片海马区N-甲基-D-天冬氨酸受体1(NMDAR1)表达情况。结果:与对照组相比,酒精组在目标象限停留时间显著缩短,海马区NMDAR1阳性细胞累积光密度(IOD)值明显降低(均P<0.05);与酒精组相比,治疗组在目标象限停留时间显著延长(P<0.05),海马区NMDAR1阳性细胞IOD值明显升高(均P<0.05)。结论:姜黄素对酒精中毒幼鼠学习记忆功能损害有改善作用,其机制可能与增加幼鼠海马区NMDAR1表达有关。
Objective:To investigate the effect and mechanisms of curcumin on learning and memory abilities in alcoholic young rats.Methods:Thirty-two male 3-week-old SD rats(50-70 g)were randomly divided into alcohol group,control group,treatment group and NaOH group,with 8 rats in each group.Alcoholism model was made by intragastric administration of 52%ethanol solution[10 mL/(kg·d)].The rats in the control group were given 52%ethanol solution via gavage and the same amount of saline.The rats in the treatment group were given 52%ethanol solution and then intraperitoneal injection of curcumin NaOH solution[100 mg/(kg·d)],and rats in the NaOH group were given intraperitoneal injection with the same concentration of NaOH only.After 7 days,Morris water maze was used to observe learning and memory function of each group rats.After 13 days,the expression of NMDAR1 in coronal section of hippocampus was observed by immunohistochemistry.Results:Compared with the control group,the time spent in the target quadrant in the alcohol group was significantly reduced,and IOD value of NMDAR1 positive cells in the hippocampus was decreased(P<0.05).Compared with the alcohol group,the time spent in the target quadrant in the treatment group was significantly increased,and IOD value of NMDAR1 positive cells in the hippocampus was increased(P<0.05).Conclusion:Curcumin could improve the learning and memory abilities of young rats with alcoholism,and the mechanism might be related to the increase of NMDAR1 expression in the hippocampus.
作者
魏苗
王有鹏
窦龙姣
黄若兰
余鸿
Wei Miao;Wang Youpeng;Dou Longjiao;Huang Ruolan;Yu Hong(Department of Histology and Embryology,Southwest Medical University,Luzhou 64600,China)
出处
《广西医科大学学报》
CAS
2019年第12期1915-1918,共4页
Journal of Guangxi Medical University
基金
2018年国家级大学生创新创业训练计划项目资助项目(No.201816032009)
