摘要
采用大孔树脂纯化大青叶黄酮提取物后,比较提取物与纯化物对小鼠运动耐力的影响。以吸附率、解吸率及产物中总黄酮含量为评价指标,优化大青叶黄酮提取物的纯化工艺条件,并测定小鼠的力竭游泳时间和相关抗疲劳生化指标。结果表明,采取AB-8大孔树脂纯化大青叶总黄酮提取物的最佳工艺条件为:上样浓度1.0 mg/mL、上样液pH 3.5、上样体积50 mL、上样流速1.5 mL/min、乙醇溶液体积分数70%、洗脱液用量80 mL、洗脱流速1.0 mL/min。该条件下所得产物的总黄酮含量为65.0%,约为纯化前3.3倍。与空白对照组和提取对照组相比,大青叶黄酮纯化物有助于增长小鼠的运动时间,减少乳酸、尿素氮在体内的累积与丙二醛的生成,提高肝组织中谷胱甘肽过氧化物酶活力,具有良好的抗运动疲劳活性。
After purifying the flavonoid extracts from Isatidis Folium by macroporous resin,the effects of the extracts and the purified substances on exercise endurance in mice were compared.The adsorption rate,desorption rate,and the total flavonoids in products were selected as the evaluation indicators,and the purified conditions of total flavonoids extract from Isatidis Folium were optimized.The exhaustion swimming time and related anti-fatigue biochemical indices of mice were measured.The results showed that the optimum purified conditions of total flavonoids from Isatidis Folium with AB-8 macroporous resin were as follows:loading sample concentration,1.0 mg/mL;pH value of loading solution,3.5;loading sample volume,50 mL;loading flow rate,1.5 mL/min;ethanol solution with a volume fraction of 70%;eluent volume,80 mL,and elution flow rate,1.0 mL/min.After purification by these conditions,the contents of total flavonoids in the purified product increased to 65.0%,which was about 3.3 times higher than that before being purified.Compared with the blank control group and extract group,Isatidis Folium flavonoids after purifying helped to increase the exercise time of mice,reduce the accumulation of lactic acid and urea nitrogen in the body,decrease the generation of malondialdehyde and increase the activity of the antioxidant enzyme(glutathione peroxidase)in liver tissue,indicating that it has good anti-exercise fatigue activity.
作者
翟兆峰
王延宝
ZHAI Zhao-feng;WANG Yan-bao(Faculty of Education,Qufu Normal University,Qufu 273165,China;Institute of Physical Education,Jining University,Qufu 273155,China;Experimental Teaching and Equipment Management Center,Jining University,Qufu 273155,China)
出处
《化学试剂》
CAS
北大核心
2023年第10期88-94,共7页
Chemical Reagents
基金
山东省社会科学规划研究项目(19CTYJ08)
山东省社会科学普及应用研究项目(2019-SKZC-12)。
关键词
大青叶
总黄酮
分离纯化
运动耐力
大孔树脂
isatidis folium
total flavonoids
isolation and purification
exercise endurance
macroporous resin
