摘要
【目的】建立小果甜柿组培快繁技术体系,实现小果甜柿组培苗的工厂化生产。【方法】以小果甜柿带芽嫩枝茎段为外植体,研究了外植体取材时间、灭菌方法、植物生长调节剂配比对其组织培养过程中外植体消毒、启动培养、继代培养、生根培养的影响。【结果】适宜小果甜柿外植体取材的时间为4月、6月;选择75%酒精10 s+0.1%氯化汞8 min的组合进行消毒,外植体成活率达76.67%;以(1/2N)MS+2.5 mg·L^(-1)ZT+0.1 mg·L^(-1)NAA为启动培养基,外植体萌芽率达78.33%;以(1/2N)MS+2.0 mg·L^(-1)ZT+0.1 mg·L^(-1)NAA为继代培养基,平均增殖系数在1.77以上;以1/2 MS+1.0 mg·L^(-1)IBA为生根培养基,生根率达74.73%,平均根数3.33条,平均根长2.60 cm。【结论】构建了小果甜柿组培快繁技术体系,为小果甜柿组培苗的工厂化生产提供参考依据。
【Objective】Persimmon(Diospyros kaki Thunb.)belongs to the genus(Diospyros Linn.)of the family Ebenaceae.China is rich in persimmon germplasm resources and one of the persimmon-producing countries with the longest cultivation history,the largest cultivated area and the largest yield in the world.Persimmon fruit tastes delicious,with unique flavor and high nutritional,and medicinal value,and is favored by consumers.At present,persimmon is often divided into sweet and astringent persimmon types according to whether the fruit can be deastringent naturally on the tree after maturity.Sweet persimmon can be crisp after ripening without any artificial treatment,sweet and refreshing,and the fresh experience of goods is excellent.The convenient transportation results in a higher economic value and persimmon industry at home and abroad focus on the development of varieties.At present,the lack of suitable rootstock in the sweet persimmon growing is the main reason that restricts its large scale development.Many domestic scholars have concluded that Xiaoguo Tianshi is a widely compatible and preferred rootstock for sweet persimmon.If the conventional seed and seedling breeding method is applied,the offspring traits will separate,and in this way,seedling breeding cycle is limited,and the yield is insufficient,so that it is difficult to meet the continuous growing market demand.Therefore,it is very important to use plant tissue culture technology for rapid propagation of Xiaoguo Tianshi as high-quality rootstock seedlings.Therefore,tissue culture has made great progress in persimmon crops and has been widely used in persimmon varieties and rootstock seedling breeding.However,there are few reports on tissue culture of Xiaoguo Tianshi,and because of the differences in physiological characteristics,explants,culture methods and other aspects among different persimmon crop varieties,the tissue culture and rapid propagation technology system of other persimmon varieties cannot be effectively applied.Therefore,the establishment of
作者
覃换玲
高营营
黄丽辉
陈淑媛
黄天琨
李贤高
周晓璇
关长飞
杨勇
QIN Huanling;GAO Yingying;HUANG Lihui;CHEN Shuyuan;HUANG Tiankun;LI Xiangao;ZHOU Xiaoxuan;GUAN Changfei;YANG Yong(Guangxi Plant Tissue Culture Seedling Co.LTD,Nanning 530007,Guangxi,China;College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《果树学报》
CAS
CSCD
北大核心
2023年第9期1992-2000,共9页
Journal of Fruit Science
关键词
小果甜柿
组织培养
快繁
茎段
培养基
Xiaoguo Tianshi
Tissues culture
Rapid propagation
Stem segment
Culture medium
