摘要
为明确野生黑果枸杞种质资源主要表型和品质性状遗传多样性,对48份黑果枸杞种质资源的18种主要表型、品质性状(15个数量性状、3个质量性状)进行描述性统计、主成分、相关性、聚类分析。结果表明,黑果枸杞种质资源具有丰富的遗传多样性,数量性状的遗传多样性指数在1.431~2.095,其变异系数在19%~189%,其中多样性指数最高的是果实纵径,其次是果实横径、枝粗、枝长、单枝成熟果实数和叶宽;质量性状遗传多样性指数在0.745~1.007,其中果实形状的多样性指数最高,叶片形状的多样性指数为0.948,果实颜色的多样性指数为0.745。主成分分析结果表明,前6个主成分累计贡献率达80.09%,果实纵径、果实颜色、叶面积、花色苷含量、果实横径和枝粗可作为反映黑果枸杞产量及品质的主要指标。相关性分析结果表明,果实横径、平均单果质量、单簇叶片数间呈极显著正相关关系(P<0.01),花色苷含量、果实颜色、单枝成熟果实数呈极显著正相关关系(P<0.01),果柄长与果实形状、果实颜色、花色苷含量和枝粗均呈极显著负相关关系(P<0.01)。基于种质间性状的遗传差异,48份黑果枸杞种质被聚为六大类,聚类结果与样品群落分布具有较强的相关性。综上,48份黑果枸杞种质资源主要表型、品质性状遗传多样性较丰富。
In order to study the genetic diversity of 18 phenotypic and quality traits(15 quantitative traits and 3 qualitative traits)of 48 germplasm resources of L.ruthenicum Murr.,descriptive statistics analysis,principal component analysis,correlation analysis and clustering analysis were performed.The results showed that the germplasm resources of L.ruthenicum Murr.were rich in genetic diversity.The genetic diversity index of quantitative traits was 1.431—2.095,and the variation coefficient was 19%—189%.The highest diversity index was longitudinal diameter of fruit,followed by transverse diameter of fruit,branch diameter,branch length,number of ripe fruits per branch and leaf width;The genetic diversity index of quality traits was 0.745—1.007,the fruit shape had the highest diversity index,the diversity index of leaf shape was 0.948,fruit color diversity index was 0.745.The results of the first six principal component analysis showed that longitudinal diameter of fruit,fruit color,leaf area,anthocyanin content,transverse diameter of fruit and branch diameter could be used as the main indexes to reflect the yield and quality of L.ruthenicum Murr.,with a cumulative contribution rate of 80.09%.The correlation analysis found that 15 pairs of traits reached the highly significant correlation level(P<0.01),and 14 pairs were positively correlated and 1 pair was negatively correlated,transverse diameter of fruit,average single fresh fruit weight and number of blades showed very significant positive correlation between each other(P<0.01),anthocyanin content,fruit color and number of ripe fruits per branch also showed very significant positive correlation between each other(P<0.01),fruit stalk length and fruit shape,fruit color,anthocyanins concentration and branch diameter showed significant negative correlation(P<0.01).Based on genetic differences of traits between germplasms,48 germplasm resources were clustered into 6 categories,and the clustering results had a strong correlation with the distribution of sample communi
作者
王晓洁
黎美霞
陶蕾
张波
何昕孺
米佳
戴国礼
徐文娣
WANG Xiaojie;LI Meixia;TAO Lei;ZHANG Bo;HE Xinru;MI Jia;DAI Guoli;XU Wendi(Institute of Lycium Barbarum Science,Ningxia Academy of Agriculture and Forestry Sciences/National Lycium Barbarum Engineering and Technology Research Center,Yinchuan 740002,China;College of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China;Innovation Team for Genetic Improvement of Economic Forest,North Minzu University,Yinchuan 750021,China)
出处
《河南农业科学》
北大核心
2023年第9期78-90,共13页
Journal of Henan Agricultural Sciences
基金
宁夏回族自治区枸杞育种专项(2013NYYZ0105)
国家自然科学基金项目(32060360)
宁夏回族自治区重点研发计划项目(2020BBF02006)。
关键词
黑果枸杞
表型性状
品质性状
遗传多样性
相关性分析
主成分分析
聚类分析
Lycium ruthenicum Murr.
Phenopytic trait
Quality trait
Genetic diversity
Correlation analysis
Principal component analysis
Clustering analysis.
