摘要
目的探讨不同严重程度肺炎支原体肺炎(MPP)患儿肺部NLRP3信号通路的变化及临床价值。方法选取2022年4月—2022年10月在湖南省人民医院进行肺泡灌洗的90例MPP患儿,并分为轻度组和重度组,分别有26和64例。选取同期该院行异物取出术后复查的10例儿童作为对照组。比较各组的临床资料,Spearman相关性分析MAP1LC3B水平与NF-KB、NLRP3水平的相关性。结果对照组MAP1LC3B、NF-κB和NLRP表达量低于MPP组(P<0.05)。轻度组与重度组性别构成、发病年龄、WBC、PLT、PCT、ALT、MP拷贝数、IgG、IgM比较,差异均无统计学意义(P>0.05)。重度组的发热时间和住院时间较轻度组长(P<0.05),CRP、LDH、D二聚体、IgA、MAP1LC3B、NF-κB和NLRP3表达量较轻度组高(P<0.05)。Spearman相关性分析显示,MAP1LC3B表达与NF-KB水平(r_(s)=0.510,P<0.05)、NLRP3水平(r_(s)=0.610,P<0.05)均呈正相关。结论自噬-NLRP3信号通路参与了MPP的发病,且与病情严重程度有关。
Objective To investigate the changes and clinical significance of NLRP3 signaling pathway in children with mycoplasma pneumoniae pneumonia(MPP)of different severity.Methods A total of 90 children with MPP who were hospitalized for alveolar lavage in our hospital from April 2022 to October 2022 were divided into mild group(26 cases)and severe group(64 cases).Meanwhile,10 children undergoing recheck after removal of foreign bodies in our hospital during the same period were set as the control group.Clinical data of the groups were compared,and the correlation of the level of MAP1LC3B with the levels of NF-KB and NLRP3 was analyzed via the Spearman method.Results Compared with the control group,the level of MAP1LC3B in the MPP group was significantly higher(P<0.05).There was no difference in the sex composition,age,WBC,PLT,PCT,ALT,and the copy number,IgM and IgG of MP between the mild group and the severe group(P>0.05),Compared with the mild group, the severe group had a longer duration of fever and length of hospital stay, and higher levels of CRP, LDH, D dimer, IgA, MAP1LC3B, NF-KB and NLRP3 (P < 0.05). Spearman correlation analysis revealed that the expression of MAP1LC3B was positively correlated with the levels of NF-KB (r_(s) =0.510, P <0.05) and NLRP3 (r_(s) =0.610, P < 0.05). Conclusions Autophagy-NLRP3 signaling pathway is involved in the pathogenesis of MPP, and is related to the severity of the disease.
作者
彭力
钟礼立
黄寒
林琳
丁小芳
陈敏
林小娟
Peng Li;Zhong Li-li;Huang Han;Lin Lin;Ding Xiao-fang;Chen Min;Lin Xiao-juan(Hunan Provincial Key Laboratory of Pediatric Respirology,First Hospital Affiliated to Hunan Normal University(Hunan Provincial People's Hospital),Changsha,Hunan 410005,China)
出处
《中国现代医学杂志》
CAS
北大核心
2023年第18期72-76,共5页
China Journal of Modern Medicine
基金
湖南省教育厅科学研究青年项目(No:22B0058)
湖南省卫健委科研课题(No:202206010013)
湖南省中医药科研课题(No:A2023057)
湖南省创新型省份建设专项(No:2019TP1043)。
关键词
肺炎支原体肺炎
儿童
自噬
mycoplasma pneumoniae pneumonia
child
autophagy
