摘要
腈水解酶是一类能够催化腈类化合物水解生成相应羧酸和氨的酶。因其独特的化学、区域和立体选择性,腈水解酶生物催化在高附加值羧酸等化学品的制备中具有重要应用,代表了原子经济和绿色化学的发展方向,成为生物有机合成中最为活跃的分支之一。本文综述了腈水解酶的来源、催化机制及基于结构功能构效关系的催化性能调控等方面研究进展,展望了腈水解酶的工业应用潜力,为发展腈水解酶设计改造方法、拓展其应用范围奠定基础。
Nitrilases are a class of enzymes catalyzing the hydrolysis of nitriles to produce corresponding carboxylic acids and ammonia.Due to their unique chemo-,regio-and enantioselectivity,nitrilase-mediated biocatalysis has demonstrated important applications in the production of high value-added carboxylic acids and other derivatives.As a major direction in terms of atomic economy and green chemistry,such biotransformation has become one of the most attractive branches in bio-organic synthesis.Herein,we first summarized the research advances in source classification and corresponding catalytic mechanisms,then discussed the regulation of catalytic properties based on structural-functional relationships.In the end,we presented the outlook for the important industrial application of nitrilases,which could be beneficial for developing the design method toward such enzymes and expanding their application scope.
作者
汤晓玲
郑仁朝
郑裕国
TANG Xiaoling;ZHENG Renchao;ZHENG Yuguo(Key Laboratory of Bioorganic Synthesis of Zhejiang Province,College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,China)
出处
《生物加工过程》
CAS
2023年第5期485-493,共9页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金(32171257)
浙江省自然科学基金(LR19B060001)。
关键词
腈水解酶
反应机制
催化性能调控
工业应用
生物有机合成
nitrilase
reaction mechanism
regulation of catalytic property
industrial application
bio-organic synthesis
