摘要
以羟基乙腈为唯一氮源,从土壤中筛选到一株腈水解酶产生菌CCZU-12,经形态观察、生理生化实验和16S rDNA序列分析,鉴定该菌为假单胞菌属(Pseudomonas sp.)。对菌株CCZU-12产腈水解酶的培养条件及催化反应条件进行优化,最适产酶培养条件为:碳源为10 g/L乙酸钠,氮源为5 g/L酵母粉,金属离子为1.0 mmol/L Mg2+,培养温度30°C,pH值7.0,接种量4%,装液量50 mL/250 mL;最适催化反应温度35°C,pH值7.0,反应120 h,羟基乙腈转化率达到98.9%。
A strain CCZU-12 with high glycolonitrile-hydrolyzing activity has been isolated using gly-colonitrile as sole nitrogen source.It was identified as Pseudomonas sp.by its morphological,physio-logical properties and 16S rDNA sequence analysis.The preferred carbon/nitrogen sources and metal ions were sodium acetate（10 g/L）,yeast extract（5 g/L）,and Mg^2＋（1.0 mmol/L）,respectively.The op-timum culture conditions were the filling volume 50 mL in 250 mL shaking flask,inoculum 4%,tem-perature 30 °C,and initial pH 7.0.After hydrolysis of glycolonitrile for 120 h,glycolic acid was ob-tained in a yield of 98.9% at the optimum reaction temperature 35 °C and pH 7.0.
出处
《微生物学通报》
CAS
CSCD
北大核心
2011年第6期825-831,共7页
Microbiology China
基金
常州市科技基金项目(No.CE20092008)
关键词
羟基乙酸
腈水解酶
假单胞菌
优化
Glycolic acid
Nitrilase
Pseudomonas sp.
Optimization
