摘要
为培育四倍体马铃薯栽培种与二倍体野生种远缘杂交优异新种质,以冀张薯8号(G8)和冀张薯12号(G12)四倍体栽培种为母本,以Z1703和Z1701二倍体野生种为父本,采用预先嫁接再杂交的方法进行组合配制,分析预先嫁接对马铃薯种间杂交亲和性及内源激素对坐果率的影响。结果表明,亲本预先嫁接,G8与G12分别与二倍体野生种进行远缘杂交,杂交后提高了各组合的坐果率和单果结实率,G8×Z1703与对照相比坐果率增加了39.19%,单果结实数提高了0.13,突破了0个实生籽,获得了栽培种与野生种远缘杂交后代。高含量赤霉素(GA_(3))和生长素(IAA)有利于坐果,而较高含量的脱落酸(ABA)抑制坐果,授粉后2~4d低含量细胞分裂素(ZR)促进坐果,后期高含量ZR促进坐果。在授粉后6d GA3含量与坐果率的相关系数高达0.922。表明马铃薯预先嫁接能够提高坐果率,促进种间杂交亲和性,内源激素对坐果率具有调控作用。
In order to cultivate excellent new germplasms of distant hybridization between tetraploid potato cultivated varieties and diploid wild varieties,tetraploid cultivated varieties(Jizhangshu 8 and Jizhangshu 12)were used as female parents and diploid wild varieties(Z1703 and Z1701)were used as male parents,the effects of pre grafting on interspecific hybridization compatibility and endogenous hormones on fruit setting rate of potato were analyzed.The results showed that the parents were pregrafted,and Jizhangshu 8 and Jizhangshu 12 were crossed with diploid wild species,respectively.After hybridization,the fruit setting rate and single fruit setting rate of each combination were improved compared with the control,the fruit setting rate of Z1703 increased by 39.19%,the number of single fruit increased by 0.13,broke through 0 seeds,and obtained the offspring of distant hybridization between cultivated and wild species.High content of GA3 and IAA were conducive to fruit setting,while high content of ABA inhibits fruit setting.Low content of ZR promotes fruit setting 2-4d after pollination,and high content of ZR promotes fruit setting in the later stage.The correlation coefficient between GA_(3) content and fruit setting rate was as high as 0.922 six days after pollination.The results showed that potato pre grafting could improve the fruit setting rate and promote interspecific hybridization compatibility,and endogenous hormones could regulate the fruit setting rate.
作者
翟鑫娜
杨佳伟
许春江
祁利潘
田再民
冯琰
尹江
龚学臣
Zhai Xinna;Yang Jiawei;Xu Chunjiang;Qi Lipan;Tian Zaimin;Feng Yan;Yin Jiang;Gong Xuechen(Hebei North University,Zhangjiakou 075000,Hebei,China)
出处
《作物杂志》
北大核心
2023年第4期182-187,共6页
Crops
基金
财政部和农业农村部:国家现代农业产业技术体系(CARS-09-P05)
河北省重点研发计划项目“马铃薯甘薯现代种业科技创新团队”(21326320D)
河北省马铃薯产业协同创新中心项目
河北省农业科技成果转化资金项目(21626316D)。
关键词
马铃薯
嫁接
亲和性
生理机制
Potato
Grafting
Affinity
Physiological mechanism
