摘要
剩余污泥是城市污水处理厂运行的主要副产物,可作为生物质能源的廉价原料.为提高剩余污泥的氢气转化率,采用间歇培养方式考察了氧化还原介体(Redox mediators,RMs)对剩余污泥发酵产氢效能的影响,并通过Illumina MiSeq测序揭示了RMs对微生物群落的影响.结果表明,在35℃及剩余污泥浓度为2.6 g·L^(-1)(MLVSS)条件下,对照系统的累计产氢量和最大产氢速率(Rmax)分别为14.3 mL·g^(-1)和5.4 mL·g^(-1)·d^(-1).当蒽醌-2-磺酸钠(AQS)加入发酵体系后,剩余污泥的产氢效率显著提高,其累计产氢量和Rmax分别比对照高出36.4%和18.5%.相反,指甲花醌、甲萘醌和氧化石墨烯(GO)导致厌氧污泥的产氢能力受到不同程度的抑制.Illumina MiSeq测序揭示了发酵系统的优势菌群存在显著差异.在对照系统中,Longilinea、Proteiniclasticum、Hyphomicrobium、Gimesia、Pirellula和Aridibacter为主要优势细菌属.AQS使得Proteiniclasticum、Pirellula、Guggenheimella和Thermomarinilinea的相对丰度分别比对照提高了46.9%、31.0%、10倍和6.8倍.冗余分析(RDA)结果表明,产氢量与Longilinea、Proteiniclasticum、Guggenheimella、Planctomicrobium和Thermomarinilinea呈正相关,而与Aridibacter、Gimesia、Pirellula和Hyphomicrobium呈负相关.
Excess sludge(ES)is the dominant by-products in municipal wastewater treatment plants and it can be used as inexpensive raw materials for bioenergy production.To improve biohydrogen conversion rate of ES,the effects of redox mediators(RMs)on the biohydrogen production from ES were investigated by batch culture.The differences of microbial community structure were revealed by Illumina MiSeq sequencing.The results showed that the accumulative biohydrogen production and maximum biohydrogen production rate(Rmax)in control at 35℃and 2.6 g·L^(-1)(MLVSS)of ES were 14.3 mL·g^(-1) and 5.4 mL·g^(-1)·d^(-1),respectively.When anthraquinone-2-sulfonic acid(AQS)was introduced to the system,the biohydrogen production of ES was significantly improved.The accumulative biohydrogen production and Rmax was higher than that in control by 36.4%and 18.5%,respectively.On the contrary,the biohydrogen production capacity of anaerobic sludge was inhibited in different levels by henna quinone,menadione and graphene oxide(GO).Illumina MiSeq sequencing revealed that the dominant microbial groups in these samples were different.Longilinea,Proteiniclasticum,Hyphomicrobium,Gimesia,Pirellula and Aridibacter were dominated in control.AQS made the relative abundance of Proteiniclasticum,Pirellula,Guggenheimella and Thermomariniline were higher than that in control by 47.3%,31.0%,10 times and 6.8 times,respectively.Redundancy analysis(RDA)indicated that biohydrogen production was positively correlated with Longilinea,Proteiniclasticum,Guggenheimella,Planctomicrobium and Thermomarinilinea,whereas it was negatively correlated with Aridibacter,Gimesia,Pirellula and Hyphomicrobium.
作者
张立国
王彤彤
刘琦
李建政
班巧英
ZHANG Liguo;WANG Tongtong;LIU Qi;LI Jianzheng;BAN Qiaoying(College of Environment and Resource,Shanxi University,Taiyuan 030006;Jinkong Electric Tashan Power Generation Shanxi Co.,Ltd,Datong 037001;State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090)
出处
《环境科学学报》
CAS
CSCD
北大核心
2023年第9期202-210,共9页
Acta Scientiae Circumstantiae
基金
山西省基础研究计划资助项目(No.202203021211314)
哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金(No.QA202137)。
