摘要
本文研究了生育力保存领域常见的冷冻载体的种类,特点和使用情况。分别对卵子胚胎、卵巢组织、精子和睾丸冷冻保存所使用的载体进行归纳整理,分析了不同的冷冻载体保存不同生物样本的发展与现状。结果表明:开放式载体与封闭式载体各有优缺点,优化封闭式载体更好的用于生物样本保存是当务之急。生物样本种类不同所使用的载体有差异,卵子与胚胎的玻璃化保存技术相对成熟,被广泛使用的是开放式Cryotop式载体;卵巢组织由于其结构的复杂性,冷冻保存尚未有统一的标准;在精子冷冻保存方面,冷冻载体多用于微量精子的冷冻保存;睾丸组织的保存仍然面临比较大的挑战。
Types,characteristics and application of common frozen carriers in the field of fertility preservation are studied in this paper.The carriers used for cryopreservation of egg embryo,ovarian tissue,sperm and testis were summarized,and the development and current situation of different cryopreservation carriers for different biological samples were analyzed.The research results show that,open carrier and closed carrier have their own advantages and disadvantages.It is urgent to optimize the closed carrier for better preservation of biological samples.Different types of biological samples use different carriers,the vitrification preservation technology of eggs and embryos is relatively mature,and the open cryotop carrier is widely used;due to the complexity of ovarian tissue structure,there is no unified standard for cryopreservation;In terms of sperm cryopreservation,frozen carriers are mostly used for the cryopreservation of micro sperm;The preservation of testicular tissue still faces great challenges.
作者
申敬
刘宝林
李维杰
周新丽
王建信
SHEN Jing;LIU Baolin;LI Weijie;ZHOU Xinli;WANG Jianxin(Institute of Biothermal Science and Technology,University of Shanghai for Science and Technology,Shanghai 200093,China;Co-innovation Center for Energy Therapy of Tumors,Shanghai 200093,China;Shanghai Technical Service Platform for Cryopreservation of Biological Resources,Shanghai 200093,China;Shanghai Origincell Biological Cryo Equipment Company Limited,Shanghai 201203,China)
出处
《制冷技术》
2023年第3期78-85,共8页
Chinese Journal of Refrigeration Technology
关键词
冷冻载体
生育力保存
玻璃化
Frozen carrier
Fertility preservation
Vitrification
