摘要
目的:测定油茶籽油中10种甾醇的含量。方法:油茶籽油经过皂化、净化、硅烷化等前处理,以胆甾烷醇为内标,采用气相色谱-质谱法测定10种甾醇的含量。结果:方法检出限为0.22~2.31 mg/100 g,定量限为0.73~7.69 mg/100 g,加标回收率为89.1%~106.8%,精密度为0.19%~2.33%(n=6)。结论:该方法测定油茶籽油中甾醇含量能有效减少干扰,结果准确可靠。
Objective:To determine the content of 10 sterols in Camellia oleifera Abel.seed oil.Method:The content of 10 sterols in Camellia oleifera Abel.seed oil was determined by gas chromatography-mass spectrometry with cholestanol as internal standard after saponification,purification and silanization.Result:The detection limit of the method was 0.22~2.31 mg/100 g,the quantitative limit was 0.73~7.69 mg/100 g,the recovery was 89.1%~106.8%,and the precision was 0.19%~2.33%(n=6).Conclusion:The method was used to determine the sterol content in Camellia oleifera Abel.seed oil can effectively reduce interference and the results are accurate and reliable.
作者
左靖文
黄诗怡
罗春花
杨学军
杨静
周峰
周建洋
ZUO Jingwen;HUANG Shiyi;LUO Chunhua;YANG Xuejun;YANG Jing;ZHOU Feng;ZHOU Jianyang(Hengyang Market Supervision Inspection and Testing Center,Hengyang 421200,China;College of Chemistry and Materials Science Hengyang Normal University,Hengyang 421008,China)
出处
《现代食品》
2023年第15期178-181,192,共5页
Modern Food
基金
湖南省自联合基金项目(2021JJ50078)
衡阳市油茶产商品质量检验重点实验室项目(202150084066)。
关键词
油茶籽油
气相色谱-质谱联用仪
甾醇
Camellia oleifera Abel.seed oil
gas chromatography-mass spectrometry
sterol
