摘要
目的:建立猪苓药材中麦角甾醇的RP-HPLC含量测定方法。方法:猪苓经甲醇超声提取后,用HPLC测定。采用Dia-mosil C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇;流速为1.0mL·min-1;检测波长为283nm;柱温30℃。结果:麦角甾醇进样量在1.64～16.4μg范围内与峰面积呈良好的线性关系(r=0.9997);麦角甾醇的平均回收率(n=5)为98.9%,RSD为1.2%。结论:采用RP-HPLC测定猪苓药材中麦角甾醇的含量,该方法灵敏、快速、准确,可作为2010年版中国药典一部增订猪苓含量测定项。
Objective ： To establish an HPLC method for the determination of ergosterol content in Polyporus umbellatus by RP - HPLC. Method：Polyporus umbeUatus was extracted with methanol by ultrasound extraction and the chromatographic procedure was carried out with Diamosil C is （250 mm × 4. 6 mm,5 μm） column, The mobile phase was 100% methanol at a flow of 1.0 mL · min^-1 and the detection wavelength was 283 nm. The temperature of column was 30℃. Results：The linear range of ergosterol was 1.64 - 16.4 μg （r = 0. 9997 ）. The average recovery （n = 5 ） of ergosterol was 98.9% and RSD was 1.2%. Conclusion： The method is. simple, accurate and reproducible can offer references for the quality control of Polyporus umbellatus.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第6期898-900,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家药典委员会《中国药典》项目(YZ-199~200)
关键词
猪苓
麦角甾醇
RP-HPLC
含量测定
Polyporus umbellatus （ Pers. ） Fries
ergosterol
RP - HPLC
assay
