摘要
目的研究三斑海马的化学成分。方法采用乙醇提取和硅胶柱色谱、Sephadex LH-20葡聚糖凝胶柱色谱、反相C18柱色谱等方法对三斑海马化学成分进行分离纯化,通过理化性质、波谱数据和文献对比,对得到的化合物进行结构鉴定。结果从三斑海马中分离得到8个化合物,分别鉴定为:L-苯丙氨酸(1)、丙氨酸(2)、肌苷(3)、胆固醇(4)、N-乙酰基酪胺(5)、尿嘧啶(6)、D-甘露醇(7)、河豚素(8)。结论化合物5、7、8为首次从三斑海马中分离得到。
Objective To study the chemical constituents of Hippocampus trimaculatus Leach. Methods After extracted with ethanol, Hippocampus trimaculatus Leach was isolated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and reversed-phase C18 column chromatography. The structures of compounds were identified by physical and chemical properties, spectral data and literature comparison. Results Eight compounds were isolated from Hippocampus trimaculatus Leach and identified as L-phenylalanine (1), alanine (2), inosine (3), cholesterol (4), N-acetyltyramine (5), uracil (6), D-mannitol (7), tetrodoine (8), respectively. Conclusion Compounds 5, 7, 8 are isolated from Hippocampus trimaculatus Leach for the first time.
作者
乔方良
夏天爽
张成中
蒋益萍
辛海量
QIAO Fangliang;XIA Tianshuang;ZHANG Chengzhong;JIANG Yiping;XIN Hailiang(Department of Pharmacognosy,School of Pharmacy,Naval Medical University,Shanghai 200433,China;Department of Chinese Medicine Identification,School of Pharmacy,Naval Medical University,Shanghai 200433,China)
出处
《药学实践与服务》
CAS
2023年第9期540-543,560,共5页
Journal of Pharmaceutical Practice and Service
基金
国家重点研发计划(2019YFC1711000)。
关键词
三斑海马
化学成分
提取分离
结构鉴定
Hippocampus trimaculatus Leach
chemical constituents
extraction and separation
structure identification
