摘要
【目的】试验旨在研究复合植物精油对产气荚膜梭菌感染肉鸡肠道屏障和抗氧化功能的影响。【方法】将288只1日龄、体重相近(38 g±2 g)、健康的罗斯308肉鸡,随机分成4个处理组,每个处理6个重复,每个重复12只鸡。对照组和感染组饲喂基础饲粮,感染+CTA(肉桂醛、百里香酚和茴香醛按照质量比为1∶3∶1组成)和感染+CAT(肉桂醛、茴香醛和百里香酚按照质量为1∶3∶1组成)组分别在基础饲粮中添加100 mg/kg复合植物精油。感染组、感染+CTA组、感染+CAT组肉鸡在14~20日龄连续经口灌服1×108CFU/mL产气荚膜梭菌(1 mL/d),对照组肉鸡灌喂等体积的疱肉培养基。试验期42 d。于试验第21、28天,采集肉鸡血液、十二指肠、空肠、回肠样品,用以检测血清二胺氧化酶(DAO)活性与血浆抗氧化指标,观察肠道形态结构,检测空肠屏障相关基因的mRNA水平和抗氧化指标。【结果】与对照组相比,产气荚膜梭菌感染提高了21、28日龄肉鸡血清DAO活性,降低了21日龄空肠与28日龄十二指肠的绒毛高度(VH)与隐窝深度(CD)的比值(VH/CD)(P<0.05)。与感染组相比,饲粮中添加CTA、CAT均可缓解因产气荚膜梭菌感染引起的血清DAO升高及回肠VH降低(P<0.05);添加CTA上调了21日龄肉鸡空肠闭合基因(occludin)、紧密连接蛋白-1(claudin-1)、黏蛋白-5ac(mucin5ac)的mRNA相对表达量,上调了28日龄肉鸡闭合连接蛋白-1(ZO-1)的mRNA相对表达量(P<0.05);添加CAT上调了28日龄肉鸡ZO-1、occludin、claudin-1、脂肪酸结合蛋白2(FABP2)的mRNA相对表达量(P<0.05);此外,添加CTA和CAT均提高了28日龄感染肉鸡血浆谷胱甘肽过氧化物酶(GSH-Px)的活性(P<0.05)。【结论】饲粮中添加复合植物精油可改善产气荚膜梭菌感染肉鸡的肠道屏障和抗氧化功能。
【Objective】This study was aimed at investigating the effect of complex plant essential oils on the intestinal barrier and antioxidant function of broiler chickens challenged with Clostridium perfringens(C.perfringens).【Method】A total of 2881-day-old healthy Ross 308 broilers were randomly divided into 4 treatment groups with 6 replicates per treatment and 12 birds each.The birds in the control and infected groups were fed the basal diet.The diet of infection+CTA and infection+CAT groups were supplemented with 100 mg/kg of complex plant essential oils,respectively.CTA was composed of cinnamaldehyde,thymol and anisaldehyde with a ratio of 1∶3∶1,and CAT was composed of cinnamaldehyde,anisaldehyde and thymol with a ratio of 1∶3∶1.Continuous oral irrigation 1×108 CFU/mL C.perfringens(1 mL/d)was administered to broilers in the infection,infection+CTA and infection+CAT groups from day 14 to day 20 of the trial.The birds in the control group were fed with an equal volume of blister meat medium.The animal trial lasted for 42 days.On days 21 and 28 of the trial,the blood,duodenum,jejunum and ileum of broilers were collected.The serum diamine oxidase(DAO)activity and the plasma levels of antioxidant-related indexes were measured.Additionally,the intestinal morphology was observed,and then the mRNA levels of jejunal barrier-related genes and antioxidant-related indexes in the jejunum were investigated.【Result】Compared with the control group,C.perfringens infection increased the serum DAO activity on days 21 and 28(P<0.05),and downregulated the ratio of villi height to crypt depth(VH/CD)of jejunum on day 21 and of duodenum on day 28 of the trial(P<0.05).Compared with the infection group,the serum activity of DAO was reduced and VH of ileum was raised with CTA and CAT treated(P<0.05).In addition,the mRNA levels of occludin,claudin-1 and mucin-5ac in the jejunum on day 21 and ZO-1 on day 28 were upregulated with CTA supplemented.The transcription levels of ZO-1,occludin,claudin-1 and FABP2 on day 28 were el
作者
孙香雪
宁楠
徐欢欢
郭双双
赵迪
王蕾
李鹏
侯永清
SUN Xiangxue;NING Nan;XU Huanhuan;GUO Shuangshuang;ZHAO Di;WANG Lei;LI Peng;HOU Yongqing(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023,China;Hubei Collaborative Innovation Center of Animal Nutrition and Feed Safety,Wuhan Polytechnic University,Wuhan 430023,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2023年第9期3573-3583,共11页
China Animal Husbandry & Veterinary Medicine
基金
湖北省重点研发计划项目(2022BBA014)。
关键词
产气荚膜梭菌
复合植物精油
肉鸡
肠道屏障
抗氧化功能
Clostridium perfringens
complex plant essential oils
broiler
intestinal barrier
antioxidant function
