摘要
目的 对中国云南省阿昌族和德昂族人群无关个体进行19个STR基因座遗传多态性调查,评估多态性数据在少数民族人群中的法医学应用价值。方法 检验154例阿昌族和405例德昂族无关个体,用Goldeneye?20A试剂盒进行扩增检测,使用ABI3130毛细管电泳仪分离扩增产物片段并进行等位基因分型。结果 阿昌族人群共检出184个等位基因,等位基因频率分布在0.003 2~0.571 4,在德昂族人群共检出212个等位基因,等位基因频率分布在0.001 2~0.556 8,TPOX、TH01、CSF1PO和D3S1358基因座多态性和识别能力较差。基因型分布均符合哈温平衡,CDP和CPE均显示有较好的识别度。结论 合并检测19个常染色体STR基因座在阿昌族和德昂族群体中具有较好的遗传多态性和个体识别能力。
Objective To study the genetic polymorphism of 19 STR loci in unrelated individuals of Achang and Deang ethnic groups in Yunnan Province,China,and to evaluate the forensic value of polymorphism data in ethnic groups.Methods 154 Achang and 405 Deang unrelated individuals were examined and amplified with Goldeneye™20A kit.ABI3130 capillary electrophoresis apparatus was used to isolate the amplified product fragments and genotype the alleles.Results A total of 184 alleles were detected in Achang population with allele frequencies ranging from 0.0032 to 0.5714,and 212 alleles were detected in De’ang population with allele frequencies ranging from 0.0012 to 0.5568,indicating poor polymorphism and identification ability of loci for TPOX,TH01,CSF1PO and D3S1358.The genotype distribution was consistent with the Hardy-Weinberg equilibrium,and both CDP and CPE showed good recognition.Conclusion The combined detection of 19 autosomal STR loci showed good genetic polymorphism and individual recognition ability in Achang and De’ang populations.
作者
李光兴
张韵梅
刘程静
王伟涛
赵凯
LI Guangxing;ZHANG Yunmei;LIU Chengjing;WANG Weitao;ZHAO Kai(Yunnan Forensic Center,Mangshi Yunnan 678400;Dept.of Criminal Investigation,Public Security Bureau of Chuxiong Yi Autonomous Prefecture,Chuxiong Yunnan 675000,China)
出处
《昆明医科大学学报》
CAS
2023年第8期8-13,共6页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81660311)。
