摘要
目的通过氯米芬联合补肾种子汤调控“同源框基因A10(HOXA-10)”表达水平,研究其影响多囊卵巢综合征(Polycysticovarysyndrome,PCOS)雌鼠子宫内膜容受性的相关机制。方法将40只SPF级SD雌性大鼠按随机数字表法分为空白对照组、模型组、氯米芬组、补肾种子汤组、氯米芬联合补肾种子汤组,每组各8只。通过丙酸睾酮+绒毛膜促性腺激素(Human chorionic gonadotropin,HCG)颈背部皮下注射SD雌鼠构建PCOS动物模型。造模成功后予以氯米芬、补肾种子汤、氯米芬联合补肾种子汤药物干预,连续14 d,期间行阴道脱落涂片,并绘制大鼠体质量增长变化曲线。大鼠处死后,Elisa检测血清性激素雌二醇(Estradiol,E2)、孕酮(Progesterone,P)、促黄体素(Luteinizinghormone,LH)、促卵泡素(Follical stimulating hormone,FSH)含量,行卵巢及子宫HE染色,免疫组化观察HOXA-10在子宫组织中表达水平。结果氯米芬组、氯米芬联合补肾种子汤组与模型组比较,性激素E2、P值升高,LH/FSH比值降低,差异有统计学意义(P<0.01);氯米芬联合补肾种子汤组与氯米芬组比较,性激素E2、P值升高,LH/FSH比值降低,差异有统计学意义(P<0.05),与补肾种子汤组比较,性激素E2值升高,差异有统计学意义(P<0.01),性激素P值升高,LH/FSH比值降低,差异有统计学意义(P<0.05)。卵巢HE染色见:卵巢囊状卵泡明显减少,卵泡形态稍欠规则,颗粒细胞层增厚,见少量黄体。子宫HE染色见:子宫内膜略显平坦,内膜增厚,子宫腺体增多,管腔轻度扩张,分泌物增多。氯米芬联合补肾种子汤组雌鼠HOXA-10表达水平明显高于模型组、氯米芬组及补肾种子汤组,差异有统计学意义(P<0.05)。以上指标数值增减及子宫、卵巢形态学改变以氯米芬联合补肾种子汤组最为明显,疗效最为显著。结论氯米芬联合补肾种子汤治疗PCOS雌鼠模型,疗效明显优于单纯使用氯米芬或补肾种子汤,其作用机制为通过下�
Objective To regulate the expression level of“homeobox gene A10(HOXA-10)”by an improved method and study the mechanism of the improved method on endometrial receptivity of female rats with polycysticovarysyndrome(PCOS).Method A total of 40 SD female rats at SPF level were randomly divided into a control group,PCOS model group,PCOS model+clomiphene group,PCOS model+kidney tonifying seed soup group,PCOS model+improvement group,with eight rats in each group.The PCOS animal model was established by neck and back subcutaneous injection of testosterone propionate and human chorionic gonadotropin(HCG).After successful mold making,the rats were intervened with clomiphene,kidney tonifying seed soup,and a modified method(clomiphene+kidney tonifying seed soup)for 14 days,during which vaginal shedding smear was taken,and the curve of weight growth of rats was drawn.After rats were killed,Elisa tested the content of serum sex hormone estradiol(E2),progesterone(P),luteinizinghormone(LH),and follicular stimulating hormone(FSH).HE staining of the ovary and uterus was performed,and the expression level of HOXA-10 in uterine tissue was observed by immunohistochemistry.Results Compared with the PCOS model+clomiphene group,PCOS model+improvement group,and PCOS model group,E2 and P values increased,and LH/FSH ratio decreased.The difference was statistically significant(P<0.01).Compared with the PCOS model+clomiphene group,E2 and P values of the PCOS model+improvement group increased,and LH/FSH ratio decreased.The difference was statistically significant(P<0.05).Compared with the PCOS model+kidney tonifying seed soup group,the value of E2 was increased(P<0.01),and that of P was increased.The ratio of LH/FSH was decreased(P<0.05).HE staining of the ovary showed that the cystic follicles of the ovary were significantly reduced,and the shape of the follicles was slightly irregular.The granulosa cell layer was thickened,and a small amount of corpus luteum was seen.HE staining of the uterus showed that the endometrium was slightly flat
作者
姚莉娟
查川淋
张雯婷
周双双
李丽梅
YAO Li-juan;ZHA Chuang-lin;ZHANG Wen-ting;ZHOU Shuang-shuang;LI Li-mei(Chuxiong Hospital of Traditional Chinese Medicine,Chuxiong Yunnan 675000;Hubei Three Gorges Vocational and Technical College,Yichang Hubei 443000)
出处
《世界中西医结合杂志》
2023年第7期1346-1351,1365,共7页
World Journal of Integrated Traditional and Western Medicine
基金
云南省应用基础研究项目-中医联合专项青年项目(202001AZ070001-101)
楚雄医药高等专科学校科研项目(2022YYXM28)。
