摘要
在无溶剂体系中,采用介孔分子筛SBA-15固定化P.fluorescens脂肪酶(PFL)拆分(R,S)-3-氯-1-苯基丙醇.结果表明,在以乙酸乙烯酯为酰基供体、水活度为0.19、温度为60℃、“记忆”pH为7.0的优化条件下反应28 h,酶促拆分转化率(c)达到49.87%,产物对映体过量值(ee_(p))达到98.73%,显示了良好的应用前景.
In solvent-free reaction system,the resolution of(R,S)-3-chloro-1-phenylpropanol was performed by P.fluorescens lipase(PFL)immobilized into Mesoporous molecular sieve SBA-15.It was found that conversion(c)of the enzymatic resolution reached 49.87%,and enantiomeric excess for the product(ee_p)reached 98.73%,under the optimized conditions of using vinyl acetate as acyl donor,water activity 0.19,“memory”pH 7.0,60℃for 28 h in solvent-free system,presenting a good promise in the enzymatic resolution of(R,S)-3-chloro-1-phenylpropanol.
作者
戴大章
姜文婷
许鹿洋
DAI Da-zhang;JIANG Wen-ting;XU Lu-yang(Key Laboratory of Medical Molecule Science and Pharmaceutical Engineering,Ministry of Industry and Information Technology,Beijing Institute of Technology,Beijing Institute of Technology,Beijing 100081,China)
出处
《分子催化》
CAS
CSCD
北大核心
2023年第4期342-347,I0001,共7页
Journal of Molecular Catalysis(China)
基金
国家自然科学基金(21276025,21808004)。
