摘要
通过硫酸二乙酯(DES)和微波复合诱变,获得遗传性状稳定的高产脂肪酶黑曲霉突变菌株CM2,酶活达174.93U/mL。对菌株CM2培养条件的优化,以橄榄油和(NH4)2SO4为最佳碳、氮源,在28℃、pH7.5的条件下,发酵CM2菌株68h,脂肪酶活为180.52U/mL。大孔树脂固定化脂肪酶在35～55℃和pH7.5～9.5之间有很好的稳定性。游离酶和固定化酶的表观失活活化能分别为52.6842kJ/mol和30.8391kJ/mol,固定化酶对温度的敏感度降低,耐受性增强。在微水相中脂肪酶催化2-辛醇和乙酸乙烯酯不对称酯交换反应中,(S)-乙酸辛酯的对映选择性高(游离酶e.e.S85.7%;固定化酶e.e.S87.7%),显示了该固定化酶在2-辛醇的手性拆分方面具有良好的应用前景。
The Aspergillus niger CM2 with high lipase activity (174.93 U/mL) was obtained by combining microwave irradiation with DES.The hereditary character of the strain CM2 was stable.The culture conditions of the strain CM2 were optimized.The lipase activity was 180.52 U/mL when the strain was cultured in the medium at 28 ℃ and pH 7.5 for 68 h,where olive oil and (NH4)2SO4 were used as the carbon and nitrogen sources,respectively.Comparing with the free lipase,the immobilized lipase adsorbed on the macroporous re...
出处
《天然产物研究与开发》
CAS
CSCD
2010年第2期209-214,231,共7页
Natural Product Research and Development
基金
湘潭市科技重点资助项目ZD20081027
教育部"大学生创新实验计划"项目081053013
关键词
黑曲霉
脂肪酶
复合诱变
固定化
酶拆分
Aspergillus niger
lipase
mutation
immobilization
resolution of 2-octanol
