摘要
采用响应面法,以超声辅助提取的方式对头花龙胆总多酚提取工艺进行优化,并检测其多酚提取物体外降糖降脂活性。结果显示:最佳提取参数为超声时间78 min,料液比1∶81(g/mL),乙醇体积分数61%。此条件下多酚得率最高,达到2.45%,与预测得率2.42%的相对偏差为1.24%,说明此模型拟合度较好。体外生物活性检测发现,其多酚提取物对葡萄糖吸收有延迟作用,同时对胆酸盐具有结合能力。
Response surface methodology was used to optimize the extraction process of total polyphenols from Gentiana ceph-alantha Franch.ex Hemsl by ultrasonic-assisted extraction,and the in vitro hypoglycemic and lipid-lowering activity of its poly-phenol extract was tested.The results showed that the optimum extraction parameters were as follows:ultrasonic time 78min,the solid-liquid ratio 1:81(g/mL),and the ethanol volume fraction 61%.Under these conditions,the yield of polyphenols was the high-est,reaching 2.45%,and the relative deviation from the predicted yield of 2.42%was 1.24%,which indicated that the model had an excellent fitting degree.Vitro bioactivity test showed that the polyphenol extract had a delayed effect on glucose absorption and a binding ability to cholate.
作者
翁贵英
李金辉
朱淼
Weng Guiying;Li Jinhui;Zhu Miao(Liupanshui Normal University,School of Biological Science and Technology,Liupanshui 553004,China;Liupanshui Normal University,School of Chemistry and Materials Engineering,Liupanshui 553004,China)
出处
《六盘水师范学院学报》
2023年第4期98-104,共7页
Journal of Liupanshui Normal University
基金
贵州省特色农业种质资源开发与利用特色重点实验室(黔教合KY字2017012)
六盘水师范学院科技创新团队“分子生物学与生物化学科技创新团队”项目(LPSSYKJTD201602)
六盘水师范学院实验教学示范中心项目(LPSSYsyjxs-fzx201702)。
关键词
头花龙胆
多酚
提取工艺
生物活性
Gentiana cephalantha Franch.ex Hemsl
polyphenols
extraction process
biological activity
