摘要
目的测定不同温度、不同储存时间下血清心肌损伤标志物心肌肌钙蛋白I、肌酸激酶同工酶、肌红蛋白含量,探究血清cTnI、CK-MB、Myo随储存时间延长的变化情况。方法收集96例心血管疾病患者中心静脉血,分别在4℃和25±3℃条件下保存,分别测定抽血即刻(0 h)、24 h、72 h、168 h、240 h的血清cTnI、CK-MB、Myo含量,探究其变化规律并进行相关性分析。结果冷藏组血清Myo变化趋势不明显,血清cTnI、CK-MB随储存时间延长逐渐下降;常温组血清Myo随储存时间延长上升,血清cTnI、CK-MB随储存时间延长逐渐下降。结论冷藏保存0~240 h及常温保存0~168 h的血液样本能够一定程度上反映死亡时心肌损伤情况,有望作为法医学诊断早期心肌损伤的新方法。
Objective To determine the contents of serum markers of myocardial injury,such as cardiac troponin I(cTnI),creatine kinase-MB(CK-MB)and myoglobin(Myo),at different temperature and storage time,and to explore the changes of these serum markers with the extension of storage time.Methods The central venous blood of 96 patients with cardiovascular diseases was collected and divided into two groups,which were stored at 4°C and 25±3°C respectively.The contents of cTnl,CK-MB and Myo in serum were measured at different storage time,including immediately(O h)after blood drawing,24 h,72 h,168 h and 240 h.The changes were explored and the correlation analysis was conducted.Results The change of serum Myo was not obvious in the refrigeration group,while the serum cTnl and CK-MB decreased with the extension of storage time.For room-temperature group,serum Myo increased with the extension of storage time,while serum cTnI and CK-MB decreased.Conclusion The blood samples stored in refrigeration for O~240 hours and at room-temperature for O~168 hours can be used to explore the myocardial injury at death,which is expected to be a new method to diagnose early myocardial injury.
作者
李佩瑾
马斌
朱伟豪
贾泽雷
尤安民
莫耀南
Li Peijin;Ma Bin;Zhu Weihao;Jia Zele;Yu Anmin;Mo Yaonan(School of Basic Medicine and Forensic Medicine,Henan University of Science and Technology,Luoyang 471000,Henan,China;Department of Nuclear Medicine,Luoyang Central Hospital affliated to Zhengzhou University,Luoyang 471000,Henan,China;School of Forensic Medicine,Hebei Medical University,Shijiazhuang 050017,Hebei,China)
出处
《中国法医学杂志》
CSCD
2023年第4期385-389,共5页
Chinese Journal of Forensic Medicine
关键词
法医病理学
心源性猝死
早期心肌损伤
心肌损伤标志物
I Forensic pathology
Sudden cardiac death
Early myocardial injury
Myocardial injury marker
