摘要
目的研究小桃儿七Helleborusthibetanus中的甾体类成分及其细胞毒性。方法采用多种现代分离色谱技术进行分离纯化,根据MS、IR和NMR等数据鉴定新化合物的结构,采用MTT法对新化合物的细胞毒活性进行筛选。结果从小桃儿七的正丁醇部位分离鉴定了2个甾体类化合物,分别鉴定为(25S)-27-O-β-D-葡萄吡喃糖基-3,25-二羟基螺甾-5(6)-烯-1β-yl-O-α-L-阿拉伯吡喃糖苷(1)和3β,14β-二羟基-3β-(2-羟基丙酰氧基)-5α-蟾酥甾-20,(22)-二烯(2),化合物2对人结肠癌HCT116细胞、人肺癌A549细胞和人肝癌HepG2细胞的半数抑制浓度(half inhibitory concentration,IC_(50))分别为(10.8±0.4)、(4.8±0.7)、(45.3±1.8)μmol/L。结论化合物1和2均为新化合物,分别命名为铁筷子苷M(thibetanosideM)和铁筷子苷N(thibetanoside N);化合物2表现出较好的细胞毒活性。
Objective To study the steroidal constituents from the roots and rhizomes of Helleborus thibetanus and their cytotoxicity.Methods A variety of modern separation chromatography techniques were used for separation and purification,and the structures of the new compounds were identified by MS,IR and NMR data,and their cytotoxic activities were screened by MTT method.Results Two new steroidal components were obtained from n-butanol fraction of ethanol extract of H.thibetanus and namely(25S)-27-O-β-D-glucopyranosyl-3,25-dihydroxyspirost-5(6)-en-1β-yl-O-α-L-arabinopyranoside(1)and 3β,14β-dihydroxy-3β-(lactate)-5α-bufa-20,(22)-dienolide(2).The half inhibitory concentration(IC_(50))of compound 2 on human colon cancer HCT116 cells,human lung cancer A549 cells and human hepatocellular carcinoma HepG2 cells,respectively,was(10.8±0.4),A549 cells(4.8±0.7)and HepG 2 cells(45.3±1.8)μmol/L.Conclusion Compounds 1 and 2 are new compounds named thibetanoside M and thibetanoside N,among which compound 2 exhibited moderate cytotoxicity.
作者
李玉泽
张东东
姜祎
邓翀
王薇
刘建利
宋小妹
LI Yu-ze;ZHANG Dong-dong;JIANG Yi;DENG Chong;WANG Wei;LIU Jian-li;SONG Xiao-mei(School of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;College of Life Science,Northwest University,Xi’an 710069,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第15期4769-4773,共5页
Chinese Traditional and Herbal Drugs
基金
陕西省中医药管理局项目(2021-02-22-017)
陕西省教育厅项目(22JK0344)
陕西中医药大学创新团队项目(2023-CXTD-05)。
关键词
小桃儿七
甾体皂苷
强心苷
细胞毒性
铁筷子苷M
铁筷子苷N
Helleborus thibetanus Franch.
steroidal saponin
bufadienolide
cytotoxicity
thibetanoside M
thibetanoside N
