摘要
目的 探讨清胰汤介导氨基末端蛋白激酶(c-jun N-terminal kinases, JNK)信号通路调控急性胰腺炎(Acute pancreatitis, AP)小鼠免疫系统对全身炎症反应及抗炎反应的作用机制。方法 将所有实验小鼠随机将其分成3组,每组30只。A组为对照组,小鼠在对应的时间点注射相同量的生理盐水;B组为重症急性胰腺炎(Severe acute pancreatitis, SAP)组,小鼠实施腹腔雨蛙素注射,以制作AP模型;C组为清胰汤相应处理的SAP组,在最后一次雨蛙素注射后1、12、24及36h分别实施清胰汤灌胃方式给药;各组在制模后分3、6和12 h各取10只小鼠,腹腔注射戊巴比妥钠麻醉后剖腹,取胰腺组织肺组织,对胰腺组织和肺组织进行镜下病理评分,检测肺组织磷酸化氨基末端蛋白激酶(Phosphorylated c-jun N-terminal kinases, p-JNK)蛋白的含量以及炎性因子肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素1β(Interleukin-1 β,IL-1β)水平。结果 3组胰腺组织、肺组织镜下病理评分比较差异存在统计学意义(P<0.05),且B组胰腺组织、肺组织镜下病理评分高于A组、C组,B组胰腺、肺部局部病变持续加重,C组与同时段B组比较,胰腺、肺部病变明显改善;WesternBlot检测p-JNK蛋白浓度水平在肺组织中的表达结果可以发现:A组小鼠的肺组织内的p-JNK蛋白仅有少量表达,B组和同时间的A组相对比,肺组织内的p-JNK浓度水平升高明显(P<0.05),C组和同时间的B组相对比,肺组织内的p-JNK浓度水平下降明显(P<0.05);酶联免疫吸附试验(Enzyme linked immunosorbent assay, ELISA)检测结果发现,B组小鼠肺组织中的TNF-α和IL-1β表达水平明显高于同时段A组、B组(P<0.05)。结论 可以确认的是针对SAP小鼠模型发生的肺损伤,清胰汤具有较好的治疗效果。早期的SAP肺损伤即会激活JNK的信号通路,转录相关的炎性因子。故清胰汤应争取早期使用,以阻止肺组织持续受损。
Objective To explore the mechanism of Qingyi Decoction(清胰汤)mediating c-jun N-terminal kinases(JNK)signaling pathway to regulate the systemic inflammatory response and anti-inflammatory response in mice with acute pancreatitis.Methods All experimental mice were randomly divided into 3 groups with 20 mice in each group.Group A(control group)was injected with the same amount of normal saline at the corresponding time point.Group B[severe acute pancreatitis(SAP)group]received intraperitoneal injection of cerulein to establish acute pancreatitis(AP)model.Group C(SAP treated with Qingyi Decoction)was treated with Qingyi Decoction by gavage at 1,12,24 and 36 hours after the last injection of rain borer.10 mice in each group were taken out at 3,6 and 12 hours after the model was made.After intraperitoneal injection of pentobarbital sodium anesthesia,the pancreas and lung tissues were taken out.The pathological scores of the pancreas and lung tissues were performed under the microscope.The contents of phosphorylated c-jun N-terminal kinases(p-JNK)protein,the inflammatory factor tumor necrosis factorα(TNF-α)and interleukin-1β(IL-1β)in the lung tissues were detected.Results The pathological scores of pancreas and lung tissue under microscope in group B were significantly higher than those in group A and group C.Westernblot analysis of p-JNK protein level in lung tissue showed that there was only a small amount of p-JNK protein expression in lung tissue of group A,p-JNK level in lung tissue of group B was significantly higher than that of group A at the same time(P<0.05),and p-JNK level in lung tissue of group C was significantly lower than that of group B at the same time(P<0.05).The results of enzyme linked immunosorbent assay(ELISA)showed that the expression levels of TNF-αand IL-1βin lung tissue of group B were significantly higher than those of group A and group B(P<0.05).Conclusion Qingyi Decoction is effective for lung injury in SAP animal model.JNK signaling pathway was activated and related inflammatory
作者
陈光华
王涛
舒波
CHEN Guanghua;WANG Tao;SHU Bo(Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan,China;College of Pharmacy,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《中华中医药学刊》
CAS
北大核心
2023年第8期249-253,共5页
Chinese Archives of Traditional Chinese Medicine
基金
四川省卫生健康委医学科技项目(21PJ104)
南充市科技局基金项目(22SXQT0091)
川北医学院附属医院院内项目(2022JC010)。
关键词
清胰汤
JNK信号通路
急性胰腺炎
小鼠
免疫系统
全身炎症反应
抗炎反应
作用机制
Qingyi Decoction(清胰汤)
JNK signal pathway
acute pancreatitis
mice
immune system
systemic inflammatory response
anti inflammatory reaction
mechanism of action
