摘要
【目的】筛选拟南芥中NBS1的互作蛋白,探究NBS1蛋白的新功能,为后续研究奠定基础。【方法】利用酵母双杂交技术筛选拟南芥c DNA文库,对得到的序列进行BLAST比对、亚细胞定位分析和基因本体注释。【结果】共有221个阳性克隆,测序后通过BLAST比对得到97个与NBS1互作的蛋白,包括膜蛋白、转运蛋白和折叠蛋白等,它们主要定位于细胞质、细胞核和叶绿体等,主要富集于4个分子功能、5个细胞组分和13个生物过程。【结论】拟南芥中与NBS1互作的蛋白在刺激响应、信号转导和细胞代谢等方面发挥着重要作用,也证明NBS1是一种多功能蛋白,但其功能及分子机制还需进一步研究。
[Purpose]To screen the interaction protein and explore the new function of NBS1 protein in Arabidopsis thaliana,laying the foundation for further research.[Methods]The yeast two-hybrid technology was used to screen the interaction proteins of NBS1 from A.thaliana cDNA library.BLAST alignment,subcellular localization analysis and gene ontology annotations were performed on the obtained sequences.[Results]There were 97 proteins interacted with NBS1 in 221 positive clones by BLAST after sequencing,including membrane proteins,transporters and folding proteins.These proteins were mainly localized in cytoplasm,nucleus and chloroplast,which were mainly enriched in four molecular functions,five cellular components and 13 biological processes.[Conclusion]The proteins interacting with NBS1 play an important role in stimulus response,signal transduction and cell metabolism.It is also confirmed that NBS1 is a multifunctional protein,but its function and molecular mechanism need further studies.
作者
吴钒漳
孙旭东
徐慧妮
WU Fanzhang;SUN Xudong;XU Huini(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2023年第4期558-565,共8页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然科学基金项目(31760582)。
