摘要
玉米实质性派生品种鉴定已成为当前种业知识产权保护的热点之一。为加快其精准高效分子鉴定技术的建立,本文利用多种类型派生品种为研究材料:京2416与京2416C(两者为遗传背景高度相近的两个自交系),京724与京72464(两者为遗传背景相近的两个自交系),以及由京724与京72464两者构建的893个DH系遗传群体等。研究分析了Maize 6H-60K芯片(包含61,214个SNP位点集合)应用于玉米派生品种鉴定的潜力。结果显示:(1)京2416与京2416C间存在829个SNP位点差异,GS值(遗传相似度)为98.7%,56.7%的差异位点集中分布在5号染色体长度约39 Mb区域内。(2)京724与京72464之间差异位点数目为4912个,GS值为90.1%,44.8%的差异位点集中分布在3号染色体上。(3)893个DH系与2个亲本京724及京72464之间的GS值分布均呈现连续性,其中与京724之间的GS值范围88.0%-97.0%,平均值为92.6%;与京72464之间的GS值范围88.3%-98.6%,平均值为94.5%。(4)893个DH系进行两两成对比较,共比较398,278对,所有DH系之间均有明确的SNP位点差异;GS值最小为87.5%,最大为99.9%,平均值为94.3%。结果表明Maize 6H-60K包含的SNP位点集能够精准评估派生、近似或极近似自交系及DH系的遗传背景,将所有材料一一鉴别明确区分开来,并具有进一步锁定与派生性状连锁标记的潜力。建议亟需基于Maize 6H-60K SNP位点集合,利用高效芯片、靶向测序等平台建立玉米实质性派生品种分子鉴定技术规程,为玉米品种知识产权保护、品种创新等提供技术支撑。
The identification of maize essentially derived variety has become the hot spot in the intellectual property protection of seed industry.In order to speed up the establishment of its accurate and efficient molecular identification technology,this article used multiple types of derived varieties as research materials:Jing 2416 and Jing 2416C(two inbred lines with highly similar genetic backgrounds),Jing 724 and Jing 72464(two inbred lines with similar genetic backgrounds),as well as 893 DH lines of genetic population constructed by Jing 724 and Jing 72464.The study researched and analyzed the potential of maize 6H-60K chip including 61,214 SNPs in identification of maize essentially derived varieties.The results were as follows:(1)There were 829 SNPs differences between Jing 2416 and Jing 2416C,the GS value(genetic similarity)was 98.7%,and 56.7%of the difference loci were concentrated in the 39 Mb region of chromosome 5.(2)There were 4912 SNPs differences between Jing 724 and Jing 72464,the GS value was 90.1%,and 44.8%of the difference loci were concentrated on chromosome 3.(3)The distribution of genetic similarity values between 893 DH lines and two parents(Jing 724 and Jing 72464)was continuous.The GS value range between 893 DH lines and Jing 724 was 88.0%-97.0%,with an average of 92.6%.The GS value range between 893 DH lines and Jing 72464 was 88.3%-98.6%,with an average of 94.5%.(4)893 DH lines were paired comparison,a total of 398,278 pairs were compared.There were specific SNP differences between all DH lines.The GS values of 893 DH lines in pairs ranged from 87.5%to 99.9%,with an average of 94.3%.Among them,the proportion of GS value≥97.0%was 8.6%,and the proportion of GS value≥99.0%was 1.3%.This study showed that maize 6H-60K SNP sets could accurately evaluate the genetic background of maize derived,similar or extremely similar inbred and DH lines,identify and distinguish all materials one by one,and had the potential to further lock the linkage markers of derived traits.It is suggested that the techni
作者
田红丽
张如养
范亚明
杨扬
张云龙
易红梅
邢锦丰
王凤格
赵久然
TIAN Hong-Li;ZHANG Ru-Yang;FAN Ya-Ming;YANG Yang;ZHANG Yun-Long;YI Hong-Mei;XING Jin-Feng;WANG Feng-Ge;ZHAO Jiu-Ran(Maize Research Institute,Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Crop DNA Fingerprinting Innovation and Utilization(Co-construction by Ministry and Province)/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding,Beijing 100097,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第11期2876-2885,共10页
Acta Agronomica Sinica
基金
国家科技创新重大项目(2022ZD04019)
北京市农林科学院创新能力建设专项(KJCX202303)资助。
