摘要
目的研究献血者和慢性乙肝患者(chronic hepatitis B,CHB)隐匿性乙型肝炎病毒感染(occult hepatitis B infection,OBI)血清学和分子生物学特征与差异。方法收集19份献血者OBI样本与19份CHB患者OBI样本,分别设为A组和B组,采用化学发光方法进行乙肝五项检测,荧光定量PCR方法进行乙型肝炎病毒(hepatitis B virus,HBV)DNA定量,巢式PCR扩增HBV S基因并测序。结果A组HBV DNA载量显著低于B组(P<0.05),两组血清学结果无明显差异,B组S基因的"a"决定簇突变率明显高于A组(P<0.05)。结论与CHB患者相比,OBI献血者病毒复制水平更低、S基因突变几率更小,血液筛查过程中使用高灵敏度的核酸检测试剂和方法才能最大限度保障输血安全。
Objective To study the serological and molecular biological characteristics and differences of occult hepatitis B infection(OBI)between blood donors and chronic hepatitis B(CHB)patients.Methods Nineteen OBI samples from blood donors and Nineteen OBI samples from CHB patients were collected,and named as group A and group B,respectively.Chemiluminescence method was used for hepatitis B five items detection.Real-time PCR method was used for HBV DNA quantification,and S gene was amplified by nested PCR and sequenced by DNA sequencing.Results The level of HBV DNA in group A was significantly lower than that in group B(P<0.05),and there were no significant differences in the serological result between the two groups.The mutation rate in the"a"determinant of S gene in group B was significantly higher than that in group A(P<0.05).Conclusions Compared with CHB patients,OBI blood donors have lower levels of viral replication and less chance of S gene mutation,and the use of highly sensitive nucleic acid detection reagents and method during blood screening can maximize the safety of blood transfusion.
作者
孙可望
王皓
刘超
姜天琪
黄象艳
Sun Kewang;Wang Hao;Liu Chao;Jiang Tianqi;Huang Xiangyan(School of Medical Laboratory,Weifang Medical College,Weifang 261041,China;Department of Transfusion Medicine,The 960 Hospital of the People’s Liberation Army(PLA)Joint Logistics Support Force(People’s Liberation Army Jinan Blood Center),Jinan 250031,China;Shandong Provincial Maternal and Child Health Care Hospital,Jinan 250014,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2023年第4期373-376,共4页
Chinese Journal of Experimental and Clinical Virology
基金
山东省自然科学基金(ZR2020MH325)。
