摘要
目的探讨结直肠癌组织中核因子E2相关因子3(NFE2L3)及其假基因1(NFE2L3P1)的水平及临床意义。方法采用实时定量PCR检测89对结直肠癌组织和癌旁组织的NFE2L3和NFE2L3P1水平并分析两者的相关性,评价NFE2L3P1水平与结直肠癌临床病理特征的关系,根据随访数据结合Kaplan-Meier生存曲线法分析不同NFE2L3P1水平对患者预后的影响。结果结直肠癌组织的NFE2L3水平高于癌旁组织(1.899±0.835 vs.1.275±0.526),NFE2L3P1水平亦高于癌旁组织(3.433±1.192 vs.1.261±0.742),差异有统计学意义(P<0.05),且NFE2L3水平和NFE2L3P1水平呈正相关(r=0.608,P<0.001)。分层分析显示组织NFE2L3P1水平与分化程度(χ^(2)=6.273,P=0.012)、淋巴结转移(χ^(2)=4.094,P=0.043)、T分期(χ^(2)=4.022,P=0.045)和TNM分期(χ^(2)=6.059,P=0.014)有关。单因素分析发现分化程度(χ^(2)=6.424,P=0.011)、肿瘤大小(χ^(2)=5.051,P=0.025)、TNM分期(χ^(2)=4.052,P=0.044)和NFE2L3P1水平(χ^(2)=6.020,P=0.014)与结直肠癌的总生存期(OS)有关,其中NFE2L3P1低水平者的中位OS为58.0(95%CI:54.551~61.449)个月,优于高水平者的52.0(95%CI:47.152~56.848)个月(P<0.05)。结论NFE2L3P1在结直肠癌中高表达,在结直肠癌的进展和预后中起着重要作用,可能是预测切除术后生存率的有用生物标志物。
Objective To investigate the levels and clinical significance of nuclear factor,erythroid 2-like 3(NFE2L3)and its pseudogene 1(NFE2L3P1)in colorectal cancer tissues.Methods Real-time quantitative PCR was used to detect the levels of NFE2L3 and NFE2L3P1 in 89 pairs of colorectal cancer tissues and adjacent tissues,and the correlation between both indices was analyzed.The relationship between NFE2L3P1 levels and clinical pathological characteristics of colorectal cancer was evaluated.Impact of different NFE2L3P1 levels on patients’prognosis was analyzed based on follow-up data combined with Kaplan-Meier survival curve method.Results NFE2L3 level in colorectal cancer tissues was higher than that in adjacent tissues(1.899±0.835 vs.1.275±0.526),and the NFE2L3P1 level was also higher than that in adjacent tissues(3.433±1.192 vs.1.261±0.742),with a statistically significant difference(P<0.05).Levels of NFE2L3 and NFE2L3P1 were positively correlated(r=0.608,P<0.001).Stratification analysis showed that differentiation degree(χ^(2)=6.273,P=0.012),lymph node metastasis(χ^(2)=4.094,P=0.043),T staging(χ^(2)=4.022,P=0.045)and TNM staging(χ^(2)=6.059,P=0.014)were related to tissue levels of NFE2L3P1.Univariate analysis revealed that degree of differentiation(χ^(2)=6.424,P=0.011),tumor size(χ^(2)=5.051,P=0.025),TNM staging(χ^(2)=4.052,P=0.044)and NFE2L3P1 levels(χ^(2)=6.020,P=0.014)were associated with the overall survival(OS)of colorectal cancer.The median OS of patients with low levels of NFE2L3P1 was 58.0(95%CI:54.551-61.449)months,which is better than 52.0(95%CI:47.152-56.848)months of patients with high levels(P<0.05).Conclusion NFE2L3P1 is highly expressed in colorectal cancer and plays an important role in the progression and prognosis of colorectal cancer.It may be a useful biomarker for predicting postoperative survival after resection.
作者
李金凤
苏楠
周丽红
赵春梅
郝亮
LI Jinfeng;SU Nan;ZHOU Lihong;ZHAO Chunmei;HAO Liang(Department of Gastrointestinal Oncology,Qinhuangdao Fourth Hospital,Qinhuangdao 066000,China)
出处
《临床肿瘤学杂志》
CAS
2023年第8期724-728,共5页
Chinese Clinical Oncology
关键词
结直肠癌
核因子E2相关因子3假基因1
预后
临床意义
Colorectal cancer
Nuclear factor,erythroid 2-like 3 pseudogene 1
Prognosis
Clinical significance
