摘要
目的探讨葛根素对子痫前期(preeclampsia,PE)大鼠胎盘组织损伤的影响及其可能的作用机制。方法将50只Wistar孕鼠随机分为对照组、模型组、葛根素低剂量、葛根素高剂量组及阿司匹林组,每组10只。除对照组外,剩余组孕鼠均于妊娠第13天(encyesis day 13,Ed 13)~Ed 21连续皮下注射亚硝基左旋精氨酸甲酯(introso L arginine methyl ester,L-NAME)50 mg·kg^(-1),以构建PE大鼠模型,对照组皮下注射等量生理盐水;葛根素低剂量、高剂量组及阿司匹林组均于Ed 15~Ed 21给药治疗,灌服给药量分别为40、80、2 mg·kg^(-1)·d^(-1),对照组及模型组大鼠灌服等体积生理盐水;均每日1次。检测Ed 15、Ed 21大鼠尾动脉血压;Bradford法检测24 h尿蛋白含量;酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、干扰素-γ(interferon-γ,IFN-γ)、白细胞介素-4(interleukin 4,IL-4)、白细胞介素-10(interleukin 10,IL-10)、白细胞介素-33(interleukin 33,IL-33)及可溶性生长刺激表达基因2蛋白(soluble growth stimulating expression gene 2 protein,sST2)水平;记录各组大鼠仔鼠数量及仔鼠存活率;分离胎盘组织并称定质量;HE染色检测胎盘组织病理学变化、胎盘滋养层细胞凋亡指数;蛋白质印迹法(Western blotting)检测各组大鼠胎盘组织中IL-33、跨膜型生长刺激表达基因2蛋白(transmembrane growth stimulation expression gene 2 protein,ST2L)的相对表达情况。结果成功构建PE大鼠模型;与对照组比较,模型组尾动脉血压及24 h尿蛋白含量升高,孕有仔鼠数量及仔鼠存活率降低,胎盘组织质量减轻,结构紊乱,毛细血管减少,细胞间隙变大,排列疏松,有明显炎性浸润及严重细胞凋亡,血清TNF-α、IFN-γ及sST2水平升高,IL-4、IL-10及IL-33水平降低,胎盘组织中IL-33蛋白相对表达水平降低,ST2L蛋白相对表达水平升高(P<0.05);与模型组比较,3个给药组尾动脉血压及
Objective To investigate the effect of puerarin on placental tissue injury in preeclampsia(PE)rats and its possible me-chanism.Methods Fifty Wistar pregnant rats were randomly divided into control group,model group,puerarin low-dose group,puerarin high-dose group and aspirin group,with 10 rats in each group.Except for the control group,PE rat model was established by continuous subcutaneous injection of 50 mg·kg^(-1) introso L arginine methyl ester(L-NAME)from the 13th day of gestation(Ed 13)to 21st day of gestation(Ed 21),and normal saline was injected subcutaneously once a day in the control group.The puerarin low-dose group,puerarin high-dose group and aspirin group were administrated with 40 mg·kg^(-1)·d^(-1),80 mg·kg^(-1)·d^(-1) and 2 mg·kg^(-1)·d^(-1),respectively,from Ed 15 to Ed 21.The control group and model group were administrated with equal volume of normal saline,once a day.The caudal arterial blood pressure of Ed 15 and Ed 21 rats was detected.Bradford method was used to detect the 24 hours urinary protein content.The serum levels of tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin 4(IL-4),interleukin 10(IL-10),interleukin 33(IL-33)and soluble growth stimulating expression gene 2 protein(sST2)were determined by enzyme-linked immunosorbent assay(ELISA).The number and survival rate of pups in each group were recorded.The placental tissue was separated and weighed.The histopathological changes of placenta were detected by HE staining.The apoptosis index of placental trophoblast cells was detected by TUNEL staining.The relative expression of IL-33 and transmembrane growth stimulation expression gene 2 protein(ST2L)in placental tissues of rats were detected by Western blotting.Results The PE rat model was successfully constructed.Compared with the control group,the tail arterial blood pressure,the 24 hours urine protein increased,the number and survival rate of offspring,the placental tissue weight,the structure disorder,and the capillaries of the model groups decreased;the cell
作者
李格琳
臧密密
付雪莲
LI Gelin;ZANG Mimi;FU Xuelian(Department of Obstetrics,Cangzhou People’s Hospital,Cangzhou 061000,China;Department of Gestational Diabetes,Cangzhou People’s Hospital,Cangzhou 061000,China;Department of Obstetrics and Gynecology,Cangzhou People’s Hospital,Cangzhou 061000,China)
出处
《西北药学杂志》
CAS
2023年第5期71-77,共7页
Northwest Pharmaceutical Journal
基金
河北省2021年度医学科学研究课题计划项目(编号:20210598)。
