摘要
目的探讨叶酸对同型半胱氨酸诱发大鼠子痫前期的预防作用及其作用机制。方法将确定妊娠的30只Wistar大鼠随机分为模型组,预防组和对照组,每组10只。除对照组外其他两组大鼠于妊娠10-19 d采用每日腹腔内注射同型半胱氨酸2 ml[Hcy,200 mg/(kg·d)]的方法建模。在建模同时,对照组每日按相同剂量的生理盐水腹腔内注射,预防组于大鼠妊娠8-19 d每日给予溶有叶酸的生理盐水2 ml灌胃,叶酸给药剂量为10 mg/(kg·d)。孕期通过测量各组血压变化、化验24 h尿蛋白定量确认子痫前期大鼠造模成功,于妊娠第19天剖宫取胎,记录仔鼠数量、死胎数,测量仔鼠体质量、身长及胎盘湿重,同时观察大鼠胎盘及肝、肾组织的异常变化,并进行组织病理学检查。结果建模前三组间大鼠的血压值、24 h尿蛋白定量差异无统计学意义;建模后模型组血压及尿蛋白定量显著高于对照组,差异有统计学意义(P <0. 01),预防组血压及尿蛋白定量低于模型组,差异有统计学意义(P <0. 05)。模型组与对照组及预防组相比,仔鼠数量减少,死胎率增高,仔鼠体质量、身长及胎盘湿重均降低,其胎盘、肝、肾均有异常组织学表现。结论叶酸可以预防子痫前期的发生,其作用机制可能与叶酸参与调节同型半胱氨酸的代谢有关。
Objective To investigate the preventive effect of folic acid against preeclampsia in rats induced by homocysteine and its mechanism. Methods Adult pregnant Wistar rats were randomly divided into three groups( n =10 in each group):model group, prevention group, and control group. The rats in model group and prevention group were injected intraperitoneally with homocysteine 2 ml[Hcy, 200 mg/(kg · d)] daily during days 10-19 after pregnancy to establish the model of preeclampsis. At the same time of establishing model, rats in control group were injected intraperitoneally with the same dose of normal saline daily. In addition, rats in prevention group were given intragastric administration with 2 ml folic acid[10 mg/(kg · d)] dissolved in the same dose of normal saline daily during days 8-19 after pregnancy. During pregnancy, the successful model of preeclampsia rat was quantified by measuring the blood pressure changes and testing 24 h urine protein in each group. At day 19 after pregnancy, the number of rat pups and stillbirths were recorded after cesarean section, and the weight and length of rat pups and the wet weight of placentas were measured. At the same time, the abnormal changes of rat placenta and liver and kidney tissues were observed, and histopathological examination was performed. Results The difference in the blood pressure and 24 h urine protein among the three groups had no statistical significance before establishing model. Compared with control group, the blood pressure and 24 h urine protein in model group were significantly increased( P <0.01).Compared with model group, blood pressure and 24 h urine protein in prevention group were significantly decreased after modeling( P <0.05). Compared with control group and prevention group, the number of rat pups were reduced, the stillbirth rate was increased, the weight of rat pups and the length of rat pups and the wet weight of placentas were decreased in model group( P <0.05).Compared with model group, the number of rat pups were increased, the sti
作者
褚晨枫
李合欣
冯勤梅
王亚荣
CHU Chenfeng;LI Hexin;FENG Qinmei;WANG Yarong(Department ofObstetrics and Gynecology, Affiliated People’s Hospital, ShanxiMedical University, Taiyuan 030000, China)
出处
《山西医科大学学报》
CAS
2019年第4期464-467,共4页
Journal of Shanxi Medical University
基金
山西省回国留学人员科研资助项目(2013-126)
